Anti-SNAP23抗体
参阅全部 SNAP23 一抗
兔多克隆抗体to SNAP23
Rabbit
适用于: WB, ICC/IF, IP, IHC-Pmore details
与反应: Mouse, Rat, Human, Recombinant fragment
Synthetic peptide corresponding to Mouse SNAP23 aa 193-210.
Sequence:
NKNRIDIANTRAKKLIDS
(Peptide available as ab4956)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3340于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (6) | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. 23kDa band represents SNAP 23 from rat brain protein extract. |
| ICC/IF | Use a concentration of 2 µg/ml. | |
| IP | (1) | Use at an assay dependent concentration. Used at a concentration of 2 ug/ml for 1 hr (see Abreview). |
| IHC-P | Use a concentration of 1 µg/ml. |
Entrez Gene: 8773 Human
Entrez Gene: 20619 Mouse
Omim: 602534 Human
SwissProt: O00161 Human
SwissProt: O09044 Mouse
Unigene: 511149 Human
Unigene: 245715 Mouse
Unigene: 14789 Rat
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Synaptosomal associated protein 23 antibody
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Synaptosomal-associated protein 23 antibody
Vesicle membrane fusion protein SNAP 23 antibody
Vesicle membrane fusion protein SNAP23 antibody
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Western blot - Anti-SNAP23 antibody (ab3340)
All lanes : Anti-SNAP23 antibody (ab3340) at 1 µg/ml
Lane 1 : Untransfected U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysates
Lane 2 : Non-specific scrambled siRNA transfected U-87 MG whole cell lysates
Lane 3 : SNAP23 KO U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysates
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.25 µg/ml
Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SNAP23.
Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)
Immunofluorescence analysis of SNAP-23 was performed using 90% confluent log phase U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells with ab3340. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3340 at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab3340)
ab3340 (1ug/ml) staining SNAP23 in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cellular membrane compartments of the lymphatic nodules.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - Anti-SNAP23 antibody (ab3340)
All lanes : Anti-SNAP23 antibody (ab3340) at 2 µg/ml
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg/ml per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.4 µg/ml
Western blot - Anti-SNAP23 antibody (ab3340)
Anti-SNAP23 antibody (ab3340) + Rat brain tissue
Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)
ICC/IF image of ab3340 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-SNAP23 antibody (ab3340)
Anti-SNAP23 antibody (ab3340) at 1 µg/ml + Recombinant Human SNAP23 protein (ab79180) at 0.001 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2 minutes
