Anti-Proteasome 20S beta 6抗体
参阅全部 Proteasome 20S beta 6 一抗
兔多克隆抗体to Proteasome 20S beta 6
Rabbit
Detects proteasome 20S beta 6 from purified bovine and human 26S proteasome samples.
适用于: ICC/IF, WBmore details
与反应: Mouse, Rat, Cow, Human
预测可用于: Xenopus laevis, Zebrafish
Synthetic peptide corresponding to Human Proteasome 20S beta 6 aa 41-58.
Sequence:
CRSGSAADTQAV/IADAVTY
WB: HeLa, BAEC whole cell lysates, HeLa, 3T3-L1, PC-3, MCF7, A549, PANC-1, Mouse Kidney, Mouse Liver and Rat Liver nuclear enriched cell lysate. ICC/IF: A431, HeLa, NIH 3T3, BAEC cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3331于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/10 - 1/100. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
Entrez Gene: 5694 Human
Entrez Gene: 19175 Mouse
Entrez Gene: 30388 Zebrafish
Omim: 600307 Human
SwissProt: P28072 Human
SwissProt: Q60692 Mouse
Unigene: 77060 Human
Unigene: 98 Mouse
Unigene: 8118 Rat
DELTA antibody
LMPY antibody
Macropain delta chain antibody
MGC5169 antibody
Multicatalytic endopeptidase complex delta chain antibody
Proteasome (prosome macropain) subunit beta type 6 antibody
Proteasome beta 6 subunit antibody
Proteasome catalytic subunit 1 antibody
Proteasome delta chain antibody
Proteasome subunit beta 6 antibody
Proteasome subunit beta type 6 antibody
Proteasome subunit beta type-6 antibody
Proteasome subunit delta antibody
Proteasome subunit Y antibody
PSB6_HUMAN antibody
PSMB6 antibody
PSY large multifunctional protease Y antibody
Y antibody
Western blot - Anti-Proteasome 20S beta 6 antibody (ab3331)
All lanes : Anti-Proteasome 20S beta 6 antibody (ab3331) at 1 µg/ml
Lane 1 : Nuclear enriched extracts from untransfected PC-3 cells
Lane 2 : Nuclear enriched extracts from non-targeting scrambled siRNA transfected PC-3 cells
Lane 3 : Nuclear enriched extracts from PSMB6 knockdown PC-3 cells
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/6000 dilution
Predicted band size: 25 kDa
Western blot - Anti-Proteasome 20S beta 6 antibody (ab3331)
All lanes : Anti-Proteasome 20S beta 6 antibody (ab3331) at 1 µg/ml
Lane 1 : HeLa nuclear enriched extract lysate
Lane 2 : 3T3-L1 nuclear enriched extract lysate
Lane 3 : PC-3 nuclear enriched extract lysate
Lane 4 : MCF7 nuclear enriched extract lysate
Lane 5 : A549 nuclear enriched extract lysate
Lane 6 : PANC-1 nuclear enriched extract lysate
Lane 7 : Mouse Kidney nuclear enriched extract lysate
Lane 8 : Mouse Liver nuclear enriched extract lysate
Lane 9 : Rat Liver nuclear enriched extract lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/6000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: 21 kDawhy is the actual band size different from the predicted?
Samples were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel and the resolved proteins were then transferred onto a Nitrocellulose membrane by iBlot® 2 Dry Blotting System. The blot was probed with the primary antibody (ab3331) and detected by chemiluminescence with secondary antibody using the iBright FL 1000. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit.
Western blot - Anti-Proteasome 20S beta 6 antibody (ab3331)
All lanes : Anti-Proteasome 20S beta 6 antibody (ab3331) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial adenocarcinoma cell line) whole cell lysate
Lane 2 : BAEC (Bovine aortic endothelial cell line) whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated secondary antibody
Predicted band size: 25 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S beta 6 antibody (ab3331)
Immunocytochemistry/Immunofluorescence analysis of Proteasome 20S beta 6 (green) showing staining in the cytoplasm and nucleus of A431 (Human epidermoid carcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3331 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S beta 6 antibody (ab3331)
Immunofluorescent analysis of Proteasome 20S Y (green) in NIH/3T3 (Mouse embryo fibroblast cell line) cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes and blocked with 3% Blocker BSA in PBS for 30 minutes at room temperature. Cells were stained with or without Proteasome 20S Y rabbit polyclonal antibody, at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at 1/1000 dilution for 1 hour at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S beta 6 antibody (ab3331)
Immunocytochemistry/Immunofluorescence analysis of Proteasome 20S beta 6 (green) showing staining in the cytoplasm and nucleus of BAEC (Bovine aortic endothelial cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3331 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S beta 6 antibody (ab3331)
Immunocytochemistry/Immunofluorescence analysis of Proteasome 20S beta 6 (green) showing staining in the cytoplasm and nucleus of HeLa (Human epithelial adenocarcinoma cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3331 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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