Anti-p75 NGF Receptor抗体[NGFR5]
参阅全部 p75 NGF Receptor 一抗
小鼠单克隆抗体[NGFR5] to p75 NGF Receptor
Mouse
适用于: ICC/IF, IHC-Pmore details
不适用于: WB
与反应: Rat, Human
预测可用于: Rabbit, Cat, Baboon不与反应: Mouse
Full length native protein (purified) corresponding to p75 NGF Receptor. Purified from A875 melanoma cell line.
Amino acids 1 - 160.
IHC-P: Human testis tissue. ICC/IF: PC-12 cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.40
Constituents: 0.0268% PBS, 0.2% BSA
浓度
100 µg 浓度为 1 mg/ml
Protein G purified
单克隆
NGFR5
IgG1
kappa
Abpromise™承诺保证使用ab3125于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (2) | Use a concentration of 5 µg/ml. |
IHC-P | (3) | Use a concentration of 1 - 2 µg/ml. |
应用说明
Is unsuitable for WB.
Entrez Gene: 4804 Human
Entrez Gene: 100349095 Rabbit
Omim: 162010 Human
SwissProt: P08138 Human
Unigene: 415768 Human
Unigene: 681726 Human
Unigene: 10980 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling p75 NGF Receptor with ab3125 at 2 µg/mL.
Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)
ICC/IF image of ab3125 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.