Anti-SREBP1 antibody [2A4]

• 产品名称

  Anti-SREBP1抗体[2A4]
  参阅全部 SREBP1 一抗

• 描述

  小鼠单克隆抗体[2A4] to SREBP1

• 宿主

  Mouse

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

• 表位

  Amino acids 301 - 407.

• 阳性对照

• WB: HeLa and HAP1 whole cell lysates.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 批次浓度范围 10 µg 浓度为 0.8 - 1 mg/ml

• 批次浓度范围 100 µg 浓度为 0.8 - 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  2A4

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab3259于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 6720 Human

• Omim: 184756 Human

• SwissProt: P36956 Human

• Unigene: 592123 Human

• 别名

• D630008H06 antibody

• Processed sterol regulatory element-binding protein 1 antibody

• SRBP1_HUMAN antibody

• SREBF 1 antibody

• SREBF1 antibody

• SREBP 1 antibody

• SREBP 1c antibody

• SREBP-1 antibody

• SREBP1 antibody

• Sterol regulatory element binding protein 1 antibody

• Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 antibody

• Sterol regulatory element binding transcription factor 1 antibody

• Sterol regulatory element-binding transcription factor 1 antibody

• ADD 1 antibody

• bHLHd1 antibody

• Class D basic helix-loop-helix protein 1 antibody

• 

  Western blot - Anti-SREBP1 antibody [2A4] (ab3259)

  All lanes : Anti-SREBP1 antibody [2A4] (ab3259) at 5 µg/ml
  
  Lane 1 : HeLa whole cell lysate
  Lane 2 : HAP1 whole cell lysate
  
  Lysates/proteins at 40 µg per lane.
  
  Performed under reducing conditions.
  
  Predicted band size: 127 kDa
  Observed band size: 125 kDawhy is the actual band size different from the predicted?
  Additional bands at: 65 kDa (possible cleavage fragment)
  
  
  
  

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab3259 and ab181602 (rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.