Anti-Alpha-synuclein抗体[syn204]
参阅全部 Alpha-synuclein 一抗
小鼠单克隆抗体[syn204] to Alpha-synuclein
Mouse
Due to sequence homology ab3309 might react with Beta synuclein
适用于: ICCmore details
与反应: Human
Recombinant full length protein corresponding to Human Alpha-synuclein.
Database link: P37840
ICC: HAP1 cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS
Protein G purified
单克隆
syn204
IgG2a
kappa
Abpromise™承诺保证使用ab3309于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC | Use a concentration of 10 µg/ml. |
Entrez Gene: 6622 Human
Omim: 163890 Human
SwissProt: P37840 Human
Unigene: 21374 Human
alphaSYN antibody
MGC105443 antibody
MGC110988 antibody
MGC127560 antibody
MGC64356 antibody
NACP antibody
Non A beta component of AD amyloid antibody
Non A4 component of amyloid antibody
Non A4 component of amyloid precursor antibody
Non-A beta component of AD amyloid antibody
Non-A-beta component of alzheimers disease amyloid , precursor of antibody
Non-A4 component of amyloid precursor antibody
Non-A4 component of amyloid, precursor of antibody
OTTHUMP00000218549 antibody
OTTHUMP00000218551 antibody
OTTHUMP00000218552 antibody
OTTHUMP00000218553 antibody
OTTHUMP00000218554 antibody
PARK 1 antibody
PARK 4 antibody
PARK1 antibody
PARK4 antibody
Parkinson disease (autosomal dominant, Lewy body) 4 antibody
Parkinson disease familial 1 antibody
SNCA antibody
Snca synuclein antibody
Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
SYN antibody
Synuclein alpha antibody
Synuclein alpha 140 antibody
Synuclein, alpha (non A4 component of amyloid precursor) antibody
SYUA_HUMAN antibody
Alpha synuclein antibody
Alpha-synuclein antibody
Alpha-synuclein, isoform NACP140 antibody
Immunocytochemistry - Anti-Alpha-synuclein antibody [syn204] (ab3309)
ab3309 staining Alpha-Synuclein in wild-type Hap1 cells (top panel) and SNCA knockout Hap1 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab3309 at 10μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
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