Anti-Neurofilament heavy polypeptide antibody [3G3]

• 产品名称

  Anti-Neurofilament heavy polypeptide抗体[3G3]
  参阅全部 Neurofilament heavy polypeptide 一抗

• 描述

  小鼠单克隆抗体[3G3] to Neurofilament heavy polypeptide

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, IHC-P, WBmore details

• 种属反应性

  与反应: Rat, Human
  

• 免疫原

  Synthetic peptide corresponding to Neurofilament heavy polypeptide aa 1-226 (C terminal).

• 阳性对照

• ICC: Rat brain microsome. WB: SH-SY5Y cells, rat brain lysates. IHC-P: Rat and human brain tissue.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: PBS, 0.1% BSA

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  3G3

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab19386于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 4744 Human

• Entrez Gene: 24587 Rat

• Omim: 162230 Human

• SwissProt: P12036 Human

• SwissProt: P16884 Rat

• Unigene: 198760 Human

• Unigene: 108194 Rat

• 别名

• Neurofilament heavy polypeptide 200kDa antibody

• Neurofilament heavy polypeptide antibody

• Neurofilament triplet H protein antibody

• NF H antibody

• NF-H antibody

• NFH antibody

• NFH_HUMAN antibody

• 200 kDa neurofilament protein antibody

• CMT2CC antibody

• Nefh antibody

• 

  Western blot - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)

  All lanes : Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386) at 1/500 dilution
  
  Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
  Lane 2 : Rat brain tissue lysates
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  All lanes : HRP-conjugated secondary antibody
  
  Predicted band size: 112 kDa
  
  
  
  

  Results show a band at ~200 kDa.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)

  Immunohistochemistry analysis of paraffin-embedded rat brain tissue (right) compared to a negative control without primary antibody (left) labelling Neurofilament Heavy Chain with ab19386. Positive staining in the cytoplasm and nucleus (right).
  

  To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Neurofilament Heavy Chain monoclonal antibody diluted in 3% BSA-PBS at 1/20 dilution overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

   

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)

  Immunohistochemistry analysis of Neurofilament, Heavy chain showing staining in the cytoplasm and axons of formalin-fixed, paraffin-embedded human brain tissue (B) and magnified section (C) compared with an isotype control (A). To expose target proteins, antigen retrieval was performed using HEIR with a buffer (pH 6.2). Tissues were probed with a Neurofilament, Heavy chain monoclonal antibody for 60 minutes at a dilution of 2 µg/mL and detection was performed using an HRP-conjugated detection system for 30 minutes followed by DAB staining.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)

  Immunofluorescence of neurofilament, heavy chain in rat cerebral cortex cultures in green.