Anti-BIRC6/APOLLON抗体
参阅全部 BIRC6/APOLLON 一抗
兔多克隆抗体to BIRC6/APOLLON
Rabbit
适用于: WB, IP, IHC-Pmore details
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide within BIRC6/APOLLON aa 4750-4850 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9NR09
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab19609于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | 1/5000 - 1/15000. Predicted molecular weight: 528 kDa. |
| IP | Use at 2-10 µg/mg of lysate. | |
| IHC-P | 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Entrez Gene: 57448 Human
Entrez Gene: 12211 Mouse
Omim: 605638 Human
SwissProt: Q9NR09 Human
SwissProt: O88738 Mouse
Unigene: 150107 Human
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Western blot - Anti-BIRC6/APOLLON antibody (ab19609)
All lanes : Anti-BIRC6/APOLLON antibody (ab19609) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK293T whole cell lysate at 50 µg
Lane 4 : NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 528 kDa
Exposure time: 3 seconds
Immunoprecipitation - Anti-BIRC6/APOLLON antibody (ab19609)
Immunoprecipitaction of HeLa cells with ab19609
Lane 1: ab19609 current lot
Lane 2: ab19609 previous lot
Lane 3: control IgG
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody (ab19609)
Immunohistochemistry analysis of formalin-fixed paraffin-embedded human breast carcinoma tissue staining BIRC6/Apollon with ab19609 at 0.2µg/ml
