Anti-SIRT2抗体
参阅全部 SIRT2 一抗
兔多克隆抗体to SIRT2
Rabbit
SIRT2 antibody (ab19388) detects SIRT2 and shows no cross-reactivity to other SIRT isoforms.
适用于: IHC-P, WB, ICC/IFmore details
与反应: Mouse, Human
Synthetic peptide corresponding to Human SIRT2 aa 377-389.
Sequence:
DEARTTEREKPQ
ICC/IF: SAOS2 transfected cell lysates WB: HeLa and MCF7 whole cell lysate; Mouse brain and liver tissue lysates IHC-P: Human heart tissue
The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Whole antiserum
The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis.
多克隆
IgG
Abpromise™承诺保证使用ab19388于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/100. | |
| WB | 1/250. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa). | |
| ICC/IF | 1/400. |
Entrez Gene: 22933 Human
Entrez Gene: 64383 Mouse
Omim: 604480 Human
SwissProt: Q8IXJ6 Human
SwissProt: Q8VDQ8 Mouse
Unigene: 466693 Human
Unigene: 272443 Mouse
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Western blot - Anti-SIRT2 antibody (ab19388)
All lanes : Anti-SIRT2 antibody (ab19388) at 1/250 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : MCF7 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, HRP conjugate at 1/5000 dilution
Predicted band size: 43 kDa
Observed band size: 30 kDawhy is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT2 antibody (ab19388)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of SIRT2 showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab19388 diluted in 3% BSA-PBS at a dilution of 1/100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody (ab19388)
Immunofluorescence staining of SIRT2 in transfected SAOS2 cells using ab19388.
