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Anti-Fibrinogen alpha chain antibody [UC45]

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  • 产品名称

    Anti-Fibrinogen alpha chain抗体[UC45]
    参阅全部 Fibrinogen Alpha Chain 一抗

  • 描述

    小鼠单克隆抗体[UC45] to Fibrinogen alpha chain

  • 宿主

    Mouse

  • 特异性

    This antibody reacts very strongly with the alpha chain of human fibrinogen, for further information please see Hogg 1983 paper quoted.

  • 经测试应用

    适用于: RIAICCFlow CytELISAmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Tissue, cells or virus corresponding to Human Fibrinogen alpha chain. Human acute monoblastic leukemia cells.

  • 常规说明


    A monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation.


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituent: 99.98% PBS

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    Purified from tissue culture supernatant.

  • Primary antibody说明

    A monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation.

  • 克隆

    单克隆

  • 克隆编号

    UC45

  • 骨髓瘤

    P3-NS1/1-Ag4-1

  • 同种型

    IgM

The Abpromise guarantee

Abpromise™承诺保证使用ab19079于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
RIA

Use at an assay dependent concentration.

ICC

Use at an assay dependent concentration.

Flow Cyt

Use 1µg for 106 cells.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

ELISA

Use at an assay dependent concentration. PubMed: 24959311

Flow Cytometry - Anti-Fibrinogen alpha chain antibody [UC45] (ab19079)

Flow Cytometry - Anti-Fibrinogen alpha chain antibody [UC45] (ab19079)

Overlay histogram showing THP1 cells stained with ab19079 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19079, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.