Anti-Fibrinogen alpha chain抗体[UC45]
参阅全部 Fibrinogen Alpha Chain 一抗
小鼠单克隆抗体[UC45] to Fibrinogen alpha chain
Mouse
This antibody reacts very strongly with the alpha chain of human fibrinogen, for further information please see Hogg 1983 paper quoted.
适用于: RIA, ICC, Flow Cyt, ELISAmore details
与反应: Mouse, Rat, Human
Tissue, cells or virus corresponding to Human Fibrinogen alpha chain. Human acute monoblastic leukemia cells.
A monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS
Immunogen affinity purified
Purified from tissue culture supernatant.
A monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation.
单克隆
UC45
P3-NS1/1-Ag4-1
IgM
Abpromise™承诺保证使用ab19079于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
RIA | Use at an assay dependent concentration. | |
ICC | Use at an assay dependent concentration. | |
Flow Cyt | Use 1µg for 106 cells. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
| |
ELISA | Use at an assay dependent concentration. PubMed: 24959311 |
Entrez Gene: 2243 Human
Entrez Gene: 14161 Mouse
Omim: 134820 Human
SwissProt: P02671 Human
SwissProt: E9PV24 Mouse
Unigene: 351593 Human
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Flow Cytometry - Anti-Fibrinogen alpha chain antibody [UC45] (ab19079)
Overlay histogram showing THP1 cells stained with ab19079 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19079, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.