Anti-PCNA抗体
参阅全部 PCNA 一抗
兔多克隆抗体to PCNA
Rabbit
适用于: IHC-P, WBmore details
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Alligator, Orangutan
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab19166于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
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SwissProt: Q9DEA3 Chicken
SwissProt: P12004 Human
SwissProt: P17918 Mouse
Unigene: 147433 Human
Unigene: 728886 Human
Unigene: 7141 Mouse
Unigene: 223 Rat
Entrez Gene: 373984 Chicken
Entrez Gene: 5111 Human
Entrez Gene: 18538 Mouse
Omim: 176740 Human
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wu:fa28e03 antibody
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Western blot - Anti-PCNA antibody (ab19166)
All lanes : Anti-PCNA antibody (ab19166) at 0.1 µg/ml
Lane 1 : HEK293T whole cell lysate
Lane 2 : NIH-3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 31 kDa
Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab19166)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling PCNA with ab19166 at 1/10000 (0.1µg/ml). Detection: DAB.
