Anti-58K Golgi蛋白抗体- Golgi Marker
参阅全部 58K Golgi protein 一抗
山羊多克隆抗体to 58K Golgi蛋白- Golgi Marker
Goat
适用于: WB, Flow Cytmore details
与反应: Human
预测可用于: Pig
Synthetic peptide:
SQLVECVPNFSEGKNQ
, corresponding to N terminal amino acids 2-17 of Human 58K Golgi protein.
Flow Cyt: HepG2 cells
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab19072于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.03 - 0.2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa). A 1 hour primary incubation is recommended for this product. | |
| Flow Cyt | Use a concentration of 10 µg/ml. |
Entrez Gene: 10841 Human
Omim: 606806 Human
SwissProt: O95954 Human
Unigene: 415846 Human
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Flow Cytometry - Anti-58K Golgi protein antibody - Golgi Marker (ab19072)
Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western blot - Anti-58K Golgi protein antibody - Golgi Marker (ab19072)
Anti-58K Golgi protein antibody - Golgi Marker (ab19072) at 0.05 µg/ml + Human liver lysate at 35 µg
Predicted band size: 59 kDa
