Anti-FEN1抗体
参阅全部 FEN1 一抗
兔多克隆抗体to FEN1
Rabbit
适用于: IP, WB, IHC-Pmore details
与反应: Human
预测可用于: Sheep, Horse, Cow, Pig, Chimpanzee, Gorilla, Chinese hamster, Orangutan, Bat
Synthetic peptide representing human Flap Structure-specific Endonuclease I encoded within exon 2 (LocusLink ID 2237). The peptide is derived from a portion of FEN1 that begins a few residues downstream from the I-domain as defined in Swiss-Prot entry P39748.
IP: HeLa whole cell lysate; WB: HeLa and HEK293T cells; IHC: human testicular seminoma tissue.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab17994于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | (1) | 1/2000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 42 kDa). |
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Entrez Gene: 2237 Human
Omim: 600393 Human
SwissProt: P39748 Human
Unigene: 409065 Human
FEN1_HUMAN antibody
Flap endonuclease 1 antibody
Flap structure specific endonuclease 1 antibody
Flap structure-specific endonuclease 1 antibody
hFEN-1 antibody
hFEN1 antibody
Maturation factor 1 antibody
MF1 antibody
Rad2 antibody
DNase IV antibody
FEN-1 antibody
FEN1 antibody
Immunoprecipitation - Anti-FEN1 antibody (ab17994)
FEN1 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab17994 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab17994 at 1 µg/mL.
Lane 1: ab17994 IP in HeLa whole cell lysate.
Lane 2: ab17994 IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds
Western blot - Anti-FEN1 antibody (ab17994)
All lanes : Anti-FEN1 antibody (ab17994) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Predicted band size: 42 kDa
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody (ab17994)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling FEN1 with ab179944 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
