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Anti-XAF1 antibody

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100ug
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产品详情
  • 产品名称

    Anti-XAF1抗体
    参阅全部 XAF1 一抗

  • 描述

    兔多克隆抗体to XAF1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IFWBIHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide (Human) (within the last 35 amino acids of C terminal end).

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab17204于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
ICC/IF(1)

Use at an assay dependent concentration. PubMed: 18485100

WB(3)

Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa. A higher 50kDa band is present at times (lot dependent). This band is not blocked by the immunogen. We do not know the identity of this extra band.

IHC-P

Use at an assay dependent concentration.

  • 数据库链接

  • 别名

    • BIRC4BP antibody

    • HSXIAPAF 1 antibody

    • HSXIAPAF1 antibody

    • XAF 1 antibody

    • XAF1 antibody

    • XAF1_HUMAN antibody

    • XIAP associated factor 1 antibody

    • XIAP-associated factor 1 antibody

    • XIAPAF1 antibody

    • BIRC 4 binding protein antibody

    • BIRC4 binding protein antibody

    • BIRC4-binding protein antibody

  • Western blot - Anti-XAF1 antibody (ab17204)

    Western blot - Anti-XAF1 antibody (ab17204)

    Lane 1 : Anti-XAF1 antibody (ab17204) at 0.5 µg/ml
    Lane 2 : Anti-XAF1 antibody (ab17204) at 1 µg/ml
    Lane 3 : Anti-XAF1 antibody (ab17204) at 2 µg/ml

    Lane 1 : Human spleen lysate
    Lanes 2-3 : Human spleen lysate

    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    Western blot analysis of XAF1 in human spleen lysate with ab17204 at

    Lane A: 0.5 ug/ml

    Lane B: 1ug/ml 

    Lane C: 2ug/ml 

    Higher 50kDa band presence is lot dependent. This band is not blocked by the immunogen.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XAF1 antibody (ab17204)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XAF1 antibody (ab17204)

    ab17204 at 2µg/ml staining XAF1 in human spleen tissue by IHC