Anti-XAF1抗体
参阅全部 XAF1 一抗
兔多克隆抗体to XAF1
Rabbit
适用于: ICC/IF, WB, IHC-Pmore details
与反应: Mouse, Rat, Human
Synthetic peptide (Human) (within the last 35 amino acids of C terminal end).
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab17204于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (1) | Use at an assay dependent concentration. PubMed: 18485100 |
WB | (3) | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa. A higher 50kDa band is present at times (lot dependent). This band is not blocked by the immunogen. We do not know the identity of this extra band. |
IHC-P | Use at an assay dependent concentration. |
Entrez Gene: 54739 Human
Entrez Gene: 327959 Mouse
Omim: 606717 Human
SwissProt: Q6GPH4 Human
SwissProt: Q5NBU8 Mouse
Unigene: 441975 Human
Unigene: 291131 Mouse
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Western blot - Anti-XAF1 antibody (ab17204)
Lane 1 : Anti-XAF1 antibody (ab17204) at 0.5 µg/ml
Lane 2 : Anti-XAF1 antibody (ab17204) at 1 µg/ml
Lane 3 : Anti-XAF1 antibody (ab17204) at 2 µg/ml
Lane 1 : Human spleen lysate
Lanes 2-3 : Human spleen lysate
Observed band size: 32 kDawhy is the actual band size different from the predicted?
Western blot analysis of XAF1 in human spleen lysate with ab17204 at
Lane A: 0.5 ug/ml
Lane B: 1ug/ml
Lane C: 2ug/ml
Higher 50kDa band presence is lot dependent. This band is not blocked by the immunogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XAF1 antibody (ab17204)
ab17204 at 2µg/ml staining XAF1 in human spleen tissue by IHC