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Anti-Cytokeratin 5 antibody [XM26]

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500uL
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产品详情
  • 产品名称

    Anti-Cytokeratin 5抗体[XM26]
    参阅全部 Cytokeratin 5 一抗

  • 描述

    小鼠单克隆抗体[XM26] to Cytokeratin 5

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-PFlow Cytmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Prokaryotic recombinant protein corresponding to 103 amino acid portion of the C-terminal region of the cytokeratin 5 molecule (Human).

  • 阳性对照

    • Skin

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    XM26

  • 同种型

    IgG1

  • 轻链类型

    kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab17130于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
IHC-P(1)

1/50 - 1/75. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cyt

1/100. 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

  • 数据库链接

  • 别名

    • Cytokeratin-5 antibody

    • Cytokeratin5 antibody

    • DDD antibody

    • DDD1 antibody

    • EBS2 antibody

    • epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody

    • K2C5_HUMAN antibody

    • K5 antibody

    • keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) antibody

    • Keratin 5 antibody

    • Keratin antibody

    • keratin complex 2, basic, gene 5 antibody

    • keratin, type II cytoskeletal 5 antibody

    • Keratin-5 antibody

    • Keratin5 antibody

    • KRT 5 antibody

    • Krt5 antibody

    • KRT5A antibody

    • type II cytoskeletal 5 antibody

    • Type-II keratin Kb5 antibody

    • 58 kDa cytokeratin antibody

    • CK-5 antibody

    • CK5 antibody

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [XM26] (ab17130)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [XM26] (ab17130)

    Formalin-fixed, paraffin-embeded human tonsil tissue stained for Cytokeratin 5 using ab17130 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0.

  • Flow Cytometry - Anti-Cytokeratin 5 antibody [XM26] (ab17130)

    Flow Cytometry - Anti-Cytokeratin 5 antibody [XM26] (ab17130)

    Overlay histogram showing A431 cells stained with ab17130 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17130, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.