Anti-Cytokeratin 5抗体[XM26]
参阅全部 Cytokeratin 5 一抗
小鼠单克隆抗体[XM26] to Cytokeratin 5
Mouse
适用于: IHC-P, Flow Cytmore details
与反应: Human
Prokaryotic recombinant protein corresponding to 103 amino acid portion of the C-terminal region of the cytokeratin 5 molecule (Human).
Skin
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA
Tissue culture supernatant
单克隆
XM26
IgG1
kappa
Abpromise™承诺保证使用ab17130于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) | 1/50 - 1/75. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt | 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Entrez Gene: 3852 Human
Omim: 148040 Human
SwissProt: P13647 Human
Unigene: 433845 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [XM26] (ab17130)
Formalin-fixed, paraffin-embeded human tonsil tissue stained for Cytokeratin 5 using ab17130 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0.
Flow Cytometry - Anti-Cytokeratin 5 antibody [XM26] (ab17130)
Overlay histogram showing A431 cells stained with ab17130 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17130, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.