Anti-Neurofibromin antibody

• 产品名称

  Anti-Neurofibromin抗体
  参阅全部 Neurofibromin 一抗

• 描述

  兔多克隆抗体to Neurofibromin

• 宿主

  Rabbit

• 经测试应用

  适用于: WB, IPmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Rat, Rabbit, Horse, Cow, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• WB: HeLa, 293T, TCMK-1 and NIH3T3 whole cell lysates. IP: HeLa whole cell lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7
  Preservative: 0.1% Sodium azide
  Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab17963于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 113577 Human

• Entrez Gene: 280877 Cow

• Entrez Gene: 4763 Human

• Entrez Gene: 18015 Mouse

• Entrez Gene: 24592 Rat

• Omim: 613113 Human

• SwissProt: P21359 Human

• SwissProt: Q04690 Mouse

• SwissProt: P97526 Rat

• 别名

• Neurofibromatosis related protein NF 1 antibody

• Neurofibromatosis related protein NF1 antibody

• neurofibromatosis type I antibody

• Neurofibromatosis-related protein NF-1 antibody

• Neurofibromin 1 antibody

• Neurofibromin truncated antibody

• Neurofibromin1 antibody

• NF 1 antibody

• NF antibody

• NF1 antibody

• NF1_HUMAN antibody

• NFNS antibody

• Type 1 Neurofibromatosis antibody

• von Recklinghausen disease neurofibromin antibody

• von Recklinghausen disease related protein VRNF antibody

• VRNF antibody

• WATS antibody

• Watson disease related protein WSS antibody

• Watson syndrome antibody

• WSS antibody

• DKFZp686J1293 antibody

• FLJ21220 antibody

• Neurofibromatosis Noonan syndrome antibody

• 

  Western blot - Anti-Neurofibromin antibody (ab17963)

  All lanes : Anti-Neurofibromin antibody (ab17963) at 0.1 µg/ml
  
  Lane 1 : HeLa whole cell lysate in NETN lysis buffer
  Lane 2 : 293T whole cell lysate in NETN lysis buffer
  Lane 3 : Jurkat whole cell lysate in NETN lysis buffer
  Lane 4 : TCMK-1 whole cell lysate in NETN lysis buffer
  Lane 5 : NIH3T3 whole cell lysate in NETN lysis buffer
  
  Lysates/proteins at 50 µg per lane.
  
  Developed using the ECL technique.
  
  Predicted band size: 319 kDa
  
  
  Exposure time: 75 seconds
  

• 

  Immunoprecipitation - Anti-Neurofibromin antibody (ab17963)

  Whole cell lysate (0.5-1.0mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

  Lane 1: IP using rabbit anti-Neurofibromin antibody.
  Lane 2: IP using ab17963 at 6 µg per reaction.
  Lane 3: Control IgG.

  For western blotting, ab17963 was used at 1 µg/ml.