Anti-Chk1抗体
参阅全部 Chk1 一抗
兔多克隆抗体to Chk1
Rabbit
适用于: IHC-P, IP, WBmore details
与反应: Human
预测可用于: Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human Chk1 aa 250-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O14757
WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab17191于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/1000 - 1/5000. | |
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/10000 - 1/25000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
Entrez Gene: 1111 Human
Omim: 603078 Human
SwissProt: O14757 Human
Unigene: 24529 Human
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Western blot - Anti-Chk1 antibody (ab17191)
All lanes : Anti-Chk1 antibody (ab17191) at 0.4 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TMCK (Mouse SV40 transformed kidney cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 54 kDa
Exposure time: 30 seconds
Immunoprecipitation - Anti-Chk1 antibody (ab17191)
Detection of Chk1 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Lane 1: Anti-Chk1 antibody (ab17191) at 6 µg/mg lysate, Lane 2: Anti-Chk1 antibody (ab 17191) at 6 µg/mg lysate, Lane 3: Control IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk1 antibody (ab17191)
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Chk1 with ab17191 at 1/1000 dilution. Detection: DAB.
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