Anti-Cytokeratin 8+18抗体[5D3]
参阅全部 Cytokeratin 8+18 一抗
小鼠单克隆抗体[5D3] to Cytokeratin 8+18
Mouse
适用于: WB, ICC/IF, IHC-P, Flow Cytmore details
与反应: Human
Tissue, cells or virus corresponding to Human Cytokeratin 8+18. Cytokeratins from the human breast carcinoma cell line MCF-7
IHC-P: Human Skin tissue. Flow cyt: MCF7 cells.
This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA
Tissue culture supernatant
This antibody stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.
单克隆
5D3
IgG1
Abpromise™承诺保证使用ab17139于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| ICC/IF | (1) | Use at an assay dependent concentration. PubMed: 21102523 |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Entrez Gene: 3856 Human
Entrez Gene: 3875 Human
Omim: 148060 Human
Omim: 148070 Human
SwissProt: P05787 Human
Unigene: 533782 Human
Unigene: 708445 Human
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)](https://www.abcam.cn/ps/products/17/ab17139/Images/ab17139-4-anti-cytokeratin-8-18-antibody-5d3-immunohistochemistry-skin-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
Paraffin embedded human skin tissue stained for Cytokeratin 8/18 using ab 17139 in immunohistochemical analysis.
![Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)](https://www.abcam.cn/ps/products/17/ab17139/Images/ab17139-25954-anti-cytokeratin-818-antibody-5d3-flow-cytometry.jpg)
Flow Cytometry - Anti-Cytokeratin 8+18 antibody [5D3] (ab17139)
Overlay histogram showing MCF7 cells stained with ab17139 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17139, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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