Anti-Apolipoprotein A I antibody [12C8]

• 产品名称

  Anti-Apolipoprotein A I抗体[12C8]
  参阅全部 Apolipoprotein A I 一抗

• 描述

  小鼠单克隆抗体[12C8] to Apolipoprotein A I

• 宿主

  Mouse

• 特异性

  No cross reactivity is seen with human apolipoprotein B.

• 经测试应用

  适用于: ELISA, WB, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length native Apolipoprotein A1, isolated from human plasma.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituents: 0.0268% PBS, 2.9% Sodium chloride

• 浓度

• 100 µl 浓度为 1.05 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  12C8

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab17278于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 335 Human

• Omim: 107680 Human

• SwissProt: P02647 Human

• Unigene: 93194 Human

• 别名

• APOA1 antibody

• APOA1_HUMAN antibody

• Apolipoprotein A-I(1-242) antibody

• Apolipoprotein A1 antibody

• Apolipoprotein AI antibody

• Apolipoprotein of high density lipoprotein antibody

• ApolipoproteinAI antibody

• Brp14 antibody

• high density lipoprotein uptake antibody

• Ltw1 antibody

• Lvtw1 antibody

• MGC117399 antibody

• Sep1 antibody

• Sep2 antibody

• Apo-AI antibody

• ApoA I antibody

• ApoA-I antibody

• 

  Western blot - Anti-Apolipoprotein A I antibody [12C8] (ab17278)

  All lanes : Anti-Apolipoprotein A I antibody [12C8] (ab17278) at 5 µg/ml
  
  Lane 1 : Human ovary tissue lysate - total protein (ab30222)
  Lane 2 : Lung (Human) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Lane 1 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  Lane 2 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Predicted band size: 24 kDa
  Observed band size: 25 kDawhy is the actual band size different from the predicted?
  Additional bands at: 260 kDa, 45 kDa, 55 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [12C8] (ab17278)

  Ab17278 staining human normal skeletal muscle. Staining is localised to the cytoplasm.
  Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.