Anti-CD8 alpha antibody [C8/144B]

• 产品名称

  Anti-CD8 alpha抗体[C8/144B]
  参阅全部 CD8 alpha 一抗

• 描述

  小鼠单克隆抗体[C8/144B] to CD8 alpha

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide corresponding to Human CD8 alpha (C terminal).
  Database link: P01732

• 阳性对照

• IHC-P: Human tonsil tissue. Flow Cyt: Human peripheral blood lymphocytes.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Preservative: 0.05% Sodium azide
  Constituents: Tissue culture supernatant, 1% BSA

• 纯度

  Tissue culture supernatant

• 克隆

  单克隆

• 克隆编号

  C8/144B

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab17147于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 925 Human

• Omim: 186910 Human

• SwissProt: P01732 Human

• Unigene: 85258 Human

• 别名

• CD8 antigen alpha polypeptide antibody

• CD8 antigen alpha polypeptide (p32) antibody

• CD8 antigen, alpha polypeptide (p32) antibody

• CD8a antibody

• CD8A antigen antibody

• CD8A molecule antibody

• CD8A_HUMAN antibody

• Leu2 antibody

• Leu2 T lymphocyte antigen antibody

• Ly 2 antibody

• Ly 35 antibody

• Ly B antibody

• Ly2 antibody

• Ly3 antibody

• Ly35 antibody

• LyB antibody

• Lyt 2.1 lymphocyte differentiation antigen (AA at 100) antibody

• LYT3 antibody

• MAL antibody

• OKT8 T cell antigen antibody

• OTTHUMP00000160760 antibody

• OTTHUMP00000160764 antibody

• OTTHUMP00000203528 antibody

• OTTHUMP00000203721 antibody

• p32 antibody

• T cell antigen Leu2 antibody

• T cell co receptor antibody

• T lymphocyte differentiation antigen T8/Leu 2 antibody

• T-cell surface glycoprotein CD8 alpha chain antibody

• T-cell surface glycoprotein Lyt 2 antibody

• T-lymphocyte differentiation antigen T8/Leu-2 antibody

• T8 T cell antigen antibody

• T8/Leu-2 T-lymphocyte differentiation antigen antibody

• alpha polypeptide (p32) antibody

• CD_antigen=CD8a antibody

• CD8 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [C8/144B] (ab17147)

  Paraffin embedded human tonsil tissue stained for CD8 T-Cell using ab17147 at 1/100 dilution in immunohistochemical analysis.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [C8/144B] (ab17147)This image is courtesy of an anonymous Abreview

  Paraffin embedded human spleen tissue stained for CD8 alpha using ab17147 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval step with Tris/EDTA pH 9.0.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [C8/144B] (ab17147)Image from Sato M et al., J Clin Med., 8, 695. Fig 3.; doi: 10.3390/jcm8050695. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

  Analysis of tumor-infiltrating lymphocytes. Immunohistochemical analysis of CD8 and CD4 (Original magnification, ×20). Typical examples of each staining are shown in both groups. Statistical analysis of each staining is shown. The number of CD8 (+) lymphocytes tends to be higher in the CS (+) group than in the CS (−) group, but the difference is not statistically significant (p = 0.052). The number of CD4 (+) lymphocytes shows no significant difference between the two groups (p = 0.28). Data represent the mean ± standard error of mean (CD4 and CD8, student’s t-test).

  Image from Sato M et al., J Clin Med., 8, 695. Fig 3.; doi: 10.3390/jcm8050695.

  Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

• 

  Flow Cytometry - Anti-CD8 alpha antibody [C8/144B] (ab17147)

  Human peripheral blood lymphocytes stained with ab17147 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab17147, 1/100 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.