Anti-Thrombin antibody [5G9]

• 产品名称

  Anti-Thrombin抗体[5G9]
  参阅全部 Thrombin 一抗

• 描述

  小鼠单克隆抗体[5G9] to Thrombin

• 宿主

  Mouse

• 特异性

  Binds to human thrombin and to a complex of human thrombin with the thrombin inhibitor hirudin.

• 经测试应用

  适用于: WB, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length native protein (purified) corresponding to Human Thrombin. Thrombin isolated from activated human plasma

• 表位

  not mapped

• 阳性对照

• purified alpha thrombin IHC-P: Human liver FFPE tissue sections.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C. Do Not Freeze.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituents: 0.0268% PBS, 2.9% Sodium chloride

• 浓度

• 100 µl 浓度为 1.06 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  5G9

• 骨髓瘤

  x63-Ag8.653

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab17199于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 2147 Human

• Omim: 176930 Human

• SwissProt: P00734 Human

• Unigene: 655207 Human

• 别名

• F2 antibody

• Factor II antibody

• Prepro coagulation factor II antibody

• Prothrombin antibody

• Prothrombin B chain antibody

• PT antibody

• RPRGL2 antibody

• Serine protease antibody

• THPH1 antibody

• THRB_HUMAN antibody

• Thrombin heavy chain antibody

• Coagulation factor II antibody

• Coagulation factor II thrombin antibody

• EC 3.4.21.5 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombin antibody [5G9] (ab17199)This image is courtesy of an Abreview submitted by Steffen Rickelt

  ab17199 staining Thrombin in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombin antibody [5G9] (ab17199)

  IHC image of Thrombin staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17199, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Western blot - Anti-Thrombin antibody [5G9] (ab17199)

  All lanes : Anti-Thrombin antibody [5G9] (ab17199) at 1 µg/ml
  
  Lane 1 : Human liver tissue lysate - total protein (ab29889)
  Lane 2 : Human Plasma Total Protein Lysate
  Lane 3 : Hep G2 nuclear extract lysate (ab14660)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 70 kDa
  Observed band size: 75 kDawhy is the actual band size different from the predicted?
  Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 12 minutes
  
  
  Thrombin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.