Anti-CD36 antibody [FA6-152]

• 产品名称

  Anti-CD36抗体[FA6-152]
  参阅全部 CD36 一抗

• 描述

  小鼠单克隆抗体[FA6-152] to CD36

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-P, Flow Cyt, IHC-Frmore details
  不适用于: WB

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human CD36. 20-Weeks-old fetal erythrocytes

• 阳性对照

• Flow cytometry: THP-1 cells; IHC-Fr: Human tonsil tissue; IHC-P: Human breast carcinoma tissue.

• 常规说明

  We have data from customer abReviews and publications indicating that this antibody is capable of detecting CD36 via western blot. However, based on customer feedback of difficulties and extensive troubleshooting using this antibody in western blot, we are removing WB as a tested application and recommend ab133625 as an alternative for use in this application.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  Constituents: PBS, 0.1% BSA

• 浓度

• 50 µg 浓度为 0.1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  0.2 µm filtered

• 克隆

  单克隆

• 克隆编号

  FA6-152

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab17044于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for WB.

• 数据库链接

• Entrez Gene: 948 Human

• Omim: 173510 Human

• SwissProt: P16671 Human

• Unigene: 120949 Human

• 别名

• CD36 antigen (collagen type I receptor, thrombospondin receptor) antibody

• CD36 antigen antibody

• CD36 molecule (thrombospondin receptor) antibody

• CD36 molecule antibody

• CD36_HUMAN antibody

• CHDS7 antibody

• Cluster determinant 36 antibody

• Collagen receptor, platelet antibody

• FAT antibody

• Fatty acid translocase antibody

• Fatty acid transport protein antibody

• Glycoprotein IIIb antibody

• GP IIIb antibody

• GP3B antibody

• GP4 antibody

• GPIIIB antibody

• GPIV antibody

• Leukocyte differentiation antigen CD36 antibody

• MGC108510 antibody

• MGC91634 antibody

• PAS 4 protein antibody

• PAS IV antibody

• PAS-4 antibody

• PASIV antibody

• Platelet collagen receptor antibody

• Platelet glycoprotein 4 antibody

• Platelet glycoprotein IV antibody

• scarb3 antibody

• Scavenger receptor class B member 3 antibody

• SR-B3 antibody

• SRB3 antibody

• Thrombospondin receptor antibody

• Adipocyte membrane protein antibody

• BDPLT10 antibody

• CD36 antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-CD36 antibody [FA6-152] (ab17044)

  Immunohistochemistry of frozen section of human tonsil tissue stained for CD36 with ab17044 at 20 µg/ml.

• 

  Flow Cytometry - Anti-CD36 antibody [FA6-152] (ab17044)

  Detection of CD36 in THP-1 cells. Red, black and blue line represent the isotype control, cells only and ab17044 at 10 μg/ml, respectively.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [FA6-152] (ab17044)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast carcinoma stained for CD36 with ab17044 at 20 µg/ml

• 

  Flow Cytometry - Anti-CD36 antibody [FA6-152] (ab17044)

  Overlay histogram showing THP1 cells stained with ab17044 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17044, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.