Anti-FBXL2抗体
参阅全部 FBXL2 一抗
山羊多克隆抗体to FBXL2
Goat
适用于: IHC-P, WBmore details
与反应: Rat, Human
预测可用于: Cow, Dog
Synthetic peptide: VFSNNDEGLINKK, corresponding to N terminal amino acids 2-14 of Human FBXL2.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab17018于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 49 kDa). 1 hour primary incubation is recommended for this product. |
Entrez Gene: 25827 Human
Omim: 605652 Human
SwissProt: Q9UKC9 Human
Unigene: 475872 Human
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Western blot - Anti-FBXL2 antibody (ab17018)
All lanes : Anti-FBXL2 antibody (ab17018) at 0.3 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 49 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot - Anti-FBXL2 antibody (ab17018)
ab17018 at 0.5µg/ml detecting FBXL2 from Human kidney lysate (35µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence. ab17018 at 0.5µg/ml detecting FBXL2 from Human kidney lysate (35µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence.
