Anti-CD44 antibody [156-3C11]

• 产品名称

  Anti-CD44抗体[156-3C11]
  参阅全部 CD44 一抗

• 描述

  小鼠单克隆抗体[156-3C11] to CD44

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, IHC-P, Flow Cytmore details

• 种属反应性

  与反应: Human, Baboon
  

• 免疫原

  Tissue, cells or virus corresponding to Human CD44. Stimulated human leukocytes

• 阳性对照

• Tonsil, HeLa cell line

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Preservative: 0.05% Sodium azide
  Constituent: 1% BSA

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  156-3C11

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab16728于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 101008690 Baboon

• Entrez Gene: 960 Human

• Omim: 107269 Human

• SwissProt: P16070 Human

• Unigene: 502328 Human

• 别名

• CD44 antibody

• CD44 antigen antibody

• CD44 molecule (Indian blood group) antibody

• CD44 molecule antibody

• CD44_HUMAN antibody

• CDw44 antibody

• CDW44 antigen antibody

• Cell surface glycoprotein CD44 antibody

• chondroitin sulfate proteoglycan 8 antibody

• CSPG8 antibody

• ECMR-III antibody

• Epican antibody

• Extracellular matrix receptor III antibody

• GP90 lymphocyte homing/adhesion receptor antibody

• HCELL antibody

• hematopoietic cell E- and L-selectin ligand antibody

• Heparan sulfate proteoglycan antibody

• Hermes antigen antibody

• homing function and Indian blood group system antibody

• HSA antibody

• HUTCH-I antibody

• HUTCH1 antibody

• HUTCHI antibody

• Hyaluronate receptor antibody

• IN antibody

• INLU-related p80 Glycoprotein antibody

• MC56 antibody

• MDU2 antibody

• MDU3 antibody

• MGC10468 antibody

• MIC4 antibody

• MUTCH I antibody

• MUTCH1 antibody

• PGP-1 antibody

• PGP-I antibody

• PGP1 antibody

• Phagocytic glycoprotein 1 antibody

• Phagocytic glycoprotein I antibody

• Soluble CD44 antibody

• LHR antibody

• BA-1 antibody

• CD 44 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] (ab16728)This image is courtesy of an anonymous Abreview

  ab16728 at 1/50 dilution staining baboon skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The sections were fixed with formaldehyde and blocked with a 1% Hydrogen peroxide / Methanol solution prior to incubation with the antibody for 1 hour. A biotinylated swine anti-goat, mouse, rabbit antibody was used as the secondary.

  See Abreview

• 

  Flow Cytometry - Anti-CD44 antibody [156-3C11] (ab16728)

  Human peripheral blood lymphocytes stained with ab16728 (red). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab16728, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] (ab16728)

  ab16728 staining human tonsil by IHC-P.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [156-3C11] (ab16728)

  ICC/IF image of ab16728 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16728, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.