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Anti-Placental alkaline phosphatase (PLAP) antibody [SP15]

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500uL
- +
1mL
- +
产品详情
  • 产品名称

    Anti-Placental alkaline phosphatase (PLAP)抗体[SP15]
    参阅全部 Placental alkaline phosphatase (PLAP) 一抗

  • 描述

    兔单克隆抗体[SP15] to Placental alkaline phosphatase (PLAP)

  • 宿主

    Rabbit

  • 特异性

    This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

  • 经测试应用

    适用于: Flow Cyt (Intra)IHC-Pmore details
    不适用于: WB

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is considered to be commercially sensitive.

  • 表位

    Not determined

  • 阳性对照

    • IHC-P: Seminoma and placenta tissues. Flow Cyt (intra): HeLa cells

  • 常规说明

    This product was switched from a hybridoma to recombinant production method on 7th March 2019.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.


    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    • - High batch-to-batch consistency and reproducibility

    • - Improved sensitivity and specificity

    • - Long-term security of supply

    • - Animal-free production

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS

  • 浓度

    • 500 µl 浓度为 0.021 mg/ml

    • 1 ml 浓度为 0.021 mg/ml

  • 纯度

    Protein A purified

  • Primary antibody说明

    This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

  • 克隆

    单克隆

  • 克隆编号

    SP15

  • 同种型

    IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab16695于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
Flow Cyt (Intra)

1/200.

IHC-P

1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 

Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

应用说明

Is unsuitable for WB.

  • 数据库链接

  • 别名

    • Alkaline phosphatase Regan isozyme antibody

    • ALP antibody

    • Alp1 antibody

    • ALPP antibody

    • FLJ61142 antibody

    • Germ-cell alkaline phosphatase antibody

    • nagao isozyme antibody

    • OTTHUMP00000164354 antibody

    • PALP antibody

    • Placental alkaline phosphatase 1 antibody

    • placental heat-stable alkaline phosphatase antibody

    • placental type antibody

    • PLAP antibody

    • PLAP-1 antibody

    • PLAP1 antibody

    • PPB1_HUMAN antibody

    • Alkaline phosphatase antibody

    • Alkaline phosphatase placental antibody

    • Alkaline phosphatase placental type antibody

  • Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

    Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

    Intracellular flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Placental alkaline phosphatase (PLAP) with purified ab16695 at 1/200 dilution (0.6µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. 

     This image was generated from the hybridoma version. 

      

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

    Human seminoma stained with ab16695.

    This image was generated from the hybridoma version.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

    Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling Alkaline Phosphatase with ab137879 at 1/50 dilution.

    This image was generated from the hybridoma version.

     

  • Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)

    Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)