Anti-Placental alkaline phosphatase (PLAP)抗体[SP15]
参阅全部 Placental alkaline phosphatase (PLAP) 一抗
兔单克隆抗体[SP15] to Placental alkaline phosphatase (PLAP)
Rabbit
This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
适用于: Flow Cyt (Intra), IHC-Pmore details
不适用于: WB
与反应: Human
预测可用于: Mouse, Rat
Recombinant fragment. This information is considered to be commercially sensitive.
Not determined
IHC-P: Seminoma and placenta tissues. Flow Cyt (intra): HeLa cells
This product was switched from a hybridoma to recombinant production method on 7th March 2019.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information see here.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
浓度
500 µl 浓度为 0.021 mg/ml
1 ml 浓度为 0.021 mg/ml
Protein A purified
This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
单克隆
SP15
IgG
Abpromise™承诺保证使用ab16695于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) | 1/200. | |
IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
应用说明
Is unsuitable for WB.
Entrez Gene: 250 Human
Entrez Gene: 18786 Mouse
Omim: 171800 Human
SwissProt: P05187 Human
Unigene: 284255 Human
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Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
Intracellular flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Placental alkaline phosphatase (PLAP) with purified ab16695 at 1/200 dilution (0.6µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.
This image was generated from the hybridoma version.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
Human seminoma stained with ab16695.
This image was generated from the hybridoma version.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)
Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling Alkaline Phosphatase with ab137879 at 1/50 dilution.
This image was generated from the hybridoma version.
Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695)