Anti-ErbB2 / HER2 antibody [SP3]

• 产品名称

  Anti-ErbB2 / HER2抗体[SP3]
  参阅全部 ErbB2 / HER2 一抗

• 描述

  兔单克隆抗体[SP3] to ErbB2 / HER2

• 宿主

  Rabbit

• 特异性

  This antibody has some non-specific staining signals in skeletal muscle tissues in IHC-P. For better sensitivity and less background, we recommend ab134182 and ab237715 as alternatives.

• 经测试应用

  适用于: IHC-Pmore details
  不适用于: Flow Cyt,ICC,IHC-Fr,IP or WB

• 种属反应性

  与反应: Human
  不与反应: Mouse

• 免疫原

  Recombinant fragment. This information is considered to be commercially sensitive.

• 表位

  Extracellular domain

• 阳性对照

• IHC-P: Human breast carcinoma tissue.

• 常规说明

  This product was switched from a hybridoma to recombinant production method on 5th March 2019.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

  Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

  
  

  This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.20
  Preservative: 0.1% Sodium azide
  Constituents: 1% BSA, PBS

• 浓度

• 批次浓度范围 100 µl 浓度为 0.101 - 0.123 mg/ml

• 批次浓度范围 1 ml 浓度为 0.101 - 0.122 mg/ml

• 500 µl 浓度为 0.122 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  SP3

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab16662于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for Flow Cyt,ICC,IHC-Fr,IP or WB.

• 数据库链接

• Entrez Gene: 2064 Human

• Omim: 164870 Human

• SwissProt: P04626 Human

• Unigene: 446352 Human

• 别名

• CD340 antigen antibody

• Cerb B2/neu protein antibody

• CerbB2 antibody

• Erb b2 receptor tyrosine kinase 2 antibody

• ErbB-2 proto-oncogene antibody

• ERBB2 antibody

• ERBB2_HUMAN antibody

• HER 2 antibody

• HER 2/NEU antibody

• HER2 antibody

• Herstatin antibody

• Human epidermal growth factor receptor 2 antibody

• Metastatic lymph node gene 19 protein antibody

• MLN 19 antibody

• MLN19 antibody

• NEU antibody

• NEU proto oncogene antibody

• Neuro/glioblastoma derived oncogene homolog antibody

• Neuroblastoma/glioblastoma derived oncogene homolog antibody

• NGL antibody

• p185erbB2 antibody

• Proto-oncogene c-ErbB-2 antibody

• Proto-oncogene Neu antibody

• Receptor tyrosine-protein kinase erbB-2 antibody

• TKR1 antibody

• Tyrosine kinase type cell surface receptor HER2 antibody

• Tyrosine kinase-type cell surface receptor HER2 antibody

• V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody

• V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody

• V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody

• V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody

• V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody

• Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody

• Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody

• C erb B2/neu protein antibody

• CD340 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (ab16662)

  Immunohistochemical analysis of formalin fixed paraffin embedded human breast cancer labelling ErbB2 with ab16662 at a concentration of 0.16 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab16662 anti-ErbB2 / HER2 antibody [SP3] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (ab16662)This image is courtesy of an Abreview submitted by Mr. Allan Wang.

  Formalin-fixed, paraffin-embedded human breast invasive ductal carcinoma tissue stained for ErbB2 / HER2 using ab16662 at 1/200 dilution in immunohistochemical analysis, followed by Goat anti Rabbit IgG HRP. Heat mediated antigen retrieval was done using a pH 8.5 buffer.

  This image was generated from the hybridoma version.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (ab16662)

  Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ErbB 2 with ab16662, followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on Human breast carcinoma, performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab16662 for 10 mins at room temperature. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 10 minutes. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).

  This image was generated from the hybridoma version.

• 

  Anti-ErbB2 / HER2 antibody [SP3] (ab16662)