Anti-nNOS (neuronal) (phospho S847)抗体
参阅全部 nNOS (neuronal) 一抗
兔多克隆抗体to nNOS (neuronal) (phospho S847)
Rabbit
This antibody shows a reduction in signal when blocked with unmodified nNOS (neuronal) peptide in WB, however when tested in ELISA, it showed less than 2% cross reactivity with the unmodified protein.
适用于: IHC-FoFr, WBmore details
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Xenopus laevis, Zebrafish, Apteronotus leptorhynchus
Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Mouse nNOS (neuronal), phosphorylated at S847.
参阅Abcam的专有抗源政策 (Peptide available as ab16981.)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab16650于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr | (1) | 1/3000. |
| WB | (2) | Use a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa). |
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SwissProt: O19132 Rabbit
Unigene: 654410 Human
Unigene: 684465 Human
Unigene: 684466 Human
Unigene: 684467 Human
Unigene: 442195 Mouse
Unigene: 44249 Mouse
Unigene: 10573 Rat
Entrez Gene: 4842 Human
Entrez Gene: 18125 Mouse
Entrez Gene: 100009243 Rabbit
Entrez Gene: 60658 Zebrafish
Omim: 163731 Human
SwissProt: P29475 Human
SwissProt: Q9Z0J4 Mouse
EC 1.14.13.39 antibody
IHPS 1 antibody
IHPS1 antibody
N-NOS antibody
NC-NOS antibody
neuronal Nitric Oxide Synthase antibody
Neuronal NOS antibody
Nitric oxide synthase , neuronal, included antibody
Nitric oxide synthase 1 (neuronal) antibody
Nitric oxide synthase 1 antibody
Nitric oxide synthase, brain antibody
Nitric oxide synthase, penile neuronal, included antibody
NNOS antibody
NO antibody
NOS 1 antibody
NOS antibody
NOS type I antibody
NOS-I antibody
NOS1 antibody
NOS1_HUMAN antibody
Peptidyl-cysteine S-nitrosylase NOS1 antibody
2310005C01Rik antibody
BNOS antibody
Constitutive NOS antibody
Western blot - Anti-nNOS (neuronal) (phospho S847) antibody (ab16650)
All lanes : Anti-nNOS (neuronal) (phospho S847) antibody (ab16650) at 1 µg/ml
Lane 1 : Forebrain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control)
Lane 4 : Forebrain (Mouse) Tissue Lysate with Mouse nNOS (neuronal) (phospho S847) peptide at 1 µg/ml
Lane 5 : Spinal Cord (Mouse) Tissue Lysate with Mouse nNOS (neuronal) (phospho S847) peptide at 1 µg/ml
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control) with Mouse nNOS (neuronal) (phospho S847) peptide at 1 µg/ml
Lane 7 : Forebrain (Mouse) Tissue Lysate with Mouse nNOS (neuronal) (unmodified) peptide at 1 µg/ml
Lane 8 : Spinal Cord (Mouse) Tissue Lysate with Mouse nNOS (neuronal) (unmodified) peptide at 1 µg/ml
Lane 9 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control) with Mouse nNOS (neuronal) (unmodified) peptide at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Observed band size: 190 kDawhy is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16650 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Western blot - Anti-nNOS (neuronal) (phospho S847) antibody (ab16650)
All lanes : Anti-nNOS (neuronal) (phospho S847) antibody (ab16650) at 1 µg/ml
Lane 1 : Forebrain (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Mouse) Tissue Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (negative control)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Observed band size: 190 kDawhy is the actual band size different from the predicted?
Exposure time: 2 minutes
Western blot - Anti-nNOS (neuronal) (phospho S847) antibody (ab16650)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Lanes 1-3 : Anti-nNOS (neuronal) (phospho S847) antibody (ab16650) at 1 µg/ml
Lane 4 : nNOS antibody at 1/2500 dilution
Lanes 1 & 4 : mouse forebrain
Lane 2 : mouse forebrain with Mouse nNOS (neuronal) (phospho S847) peptide (ab16981) at 1 µg/ml
Lane 3 : mouse forebrain with corresponding unmodified nNOS (neuronal) peptide at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 160 kDa
Observed band size: 160 kDa
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-nNOS (neuronal) (phospho S847) antibody (ab16650)This image is courtesy of Sophie Pezet, CNRS, Paris, France
Immunostaining using Rabbit polyclonal to nNOS (neuronal) (phospho S847) (ab16650) on rat brain tissue sections (30 micron free floating). ab16650 was used at a dilution of 1/3000 and incubated for 18 hours at RT (in PBS triton 0.3%). Secondary Antibody Goat anti-rabbit alexa Fluor 488 was used at a dilution of 1/1000. The image shows cytoplasmic staining of CNS neurons with ab16650 in naïve rats; the staining being observed in the soma and processes of these neurons. The staining was quenched by pre-incubation with peptide against phospho S847 (ab16981), but not by the control peptide (ab57047) indicating that ab16650 is specific for nNos phosphorylated at S847. Protocol: Rats were perfused-fixed with 4% paraformaldehyde. Tissues were post-fixed overnight in the same fixative and then cryoprotected in 20% sucrose overnight. Following embedding in OCT and freezing, tissues were cut and immunostained using the 'free floating’ technique.
