Anti-H-FABP抗体[67D3]
参阅全部 H-FABP 一抗
小鼠单克隆抗体[67D3] to H-FABP
Mouse
Ab16916 binds to human H-FABP. It shows no binding to human intestinal or liver FABP and slightly cross reacts with B-FABP.
适用于: IHC-P, IHC-Frmore details
与反应: Human
预测可用于: Mouse, Rat
Full length native protein (purified) corresponding to Human H-FABP.
Heart cells, recombinant human H-FABP This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
This antibody can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA
浓度
500 µl 浓度为 0.1 mg/ml
Protein G purified
Provided as a 0.2µm filtered antibody solution.
This antibody can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage.
单克隆
67D3
IgG1
Abpromise™承诺保证使用ab16916于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) | Use at an assay dependent concentration. |
IHC-Fr | Use at an assay dependent concentration. |
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Unigene: 388886 Mouse
Unigene: 32566 Rat
Entrez Gene: 2170 Human
Entrez Gene: 14077 Mouse
Omim: 134651 Human
SwissProt: P05413 Human
SwissProt: P11404 Mouse
Unigene: 657242 Human
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Immunohistochemistry (Frozen sections) - Anti-H-FABP antibody [67D3] (ab16916)
Immunohistochemical staining of H-FABP in human skeletal muscle tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [67D3] (ab16916)
IHC image of H-FABP staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16916, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.