Anti-GAP43抗体- Neuronal Marker
参阅全部 GAP43 一抗
兔多克隆抗体to GAP43 - Neuronal Marker
Rabbit
This antibody does not perform well in IHC-P (abreview 21090, 21089)
适用于: WB, IHC (PFA fixed)more details
不适用于: IHC-P
与反应: Mouse, Rat
预测可用于: Human, Chimpanzee
Synthetic peptide corresponding to Rat GAP43 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as ab16379)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.9 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab16053于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | Use a concentration of 1 µg/ml. Detects a band of approximately 43-53 kDa (predicted molecular weight: 43-53 kDa). |
| IHC (PFA fixed) | Use at an assay dependent concentration. |
应用说明
Is unsuitable for IHC-P.
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Unigene: 134974 Human
Unigene: 1222 Mouse
Unigene: 10928 Rat
Entrez Gene: 460599 Chimpanzee
Entrez Gene: 2596 Human
Entrez Gene: 14432 Mouse
GenBank: NP_002036 Human
Omim: 162060 Human
SwissProt: P17677 Human
SwissProt: P06837 Mouse
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Axonal membrane protein GAP 43 antibody
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Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)
All lanes : Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml
Lane 1 : spinal cord chung rat 1
Lane 2 : spinal cord chung rat 2
Lane 3 : spinal cord chung rat 3
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Dako Goat polyclonal to Rabbit IgG (HRP) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 43-53 kDa
Observed band size: 43 kDawhy is the actual band size different from the predicted?
Additional bands at: 90 kDa (possible multimer)
Immunohistochemistry (PFA fixed) - Anti-GAP43 antibody - Neuronal Marker (ab16053)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom
Immunofluorescent staining obtained with ab16053 Rabbit polyclonal to GAP43 in rat dorsal root ganglion, 2 weeks after a spinal nerve injury. The staining is localised in the cytoplasm of some lesioned neurons (arrow in B) and in many axons (arrow heads in B). Image B (X40 objective) is a higher magnification of A (X20). The staining disappeared completely when the antibody was pre-incubated with the immunising (blocking) peptide (ab16379).
Protocol details: Rats were intracardially perfused with 4% paraformaldehyde, DRG tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. IHC free floating was performed on the fixed, frozen and cut (20µm sections) rat tissue. Primary antibody ab16053 was incubated overnight at toom temperature at 1/300. Secondary antibody Alexa fluor 488 was incubated at 1/1000 for 2 hours at room temperature.
Western blot - Anti-GAP43 antibody - Neuronal Marker (ab16053)
Anti-GAP43 antibody - Neuronal Marker (ab16053) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43-53 kDa
Observed band size: 43 kDawhy is the actual band size different from the predicted?
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
