Anti-DR5抗体
参阅全部 DR5 一抗
兔多克隆抗体to DR5
Rabbit
适用于: ICC, WBmore details
与反应: Rat, Human
Synthetic peptide:
CVPEQEMEVQEPAEPTG
, corresponding to amino acids 255-270 of Rat DR5.
WB: HeLa, PC12, A549 and HEL 92.1.7 cell lysate. ICC: HeLa and HCT 116 cells
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab16329于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use a concentration of 2 - 3 µg/ml. | |
| WB | (1) | Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. |
Entrez Gene: 8795 Human
Omim: 603612 Human
SwissProt: O14763 Human
Unigene: 521456 Human
Unigene: 661668 Human
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Western blot - Anti-DR5 antibody (ab16329)
All lanes : Anti-DR5 antibody (ab16329) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC12 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP conjugated anti-rabbit
Developed using the ECL technique.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Immunocytochemistry - Anti-DR5 antibody (ab16329)
Immunocytochemistry analysis of DR5 was done on 70% confluent log phase HeLa cell. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab16329 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Western blot - Anti-DR5 antibody (ab16329)
All lanes : Anti-DR5 antibody (ab16329) at 2 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : HEL 92.1.7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ at 1/2500 dilution
Predicted band size: 58 kDa
Detected by chemiluminescence
Immunocytochemistry - Anti-DR5 antibody (ab16329)
Immunocytochemistry analysis of DR5 (TRAIL-R2) was performed using HCT 116 cells and HCT 116 treated with Thapsigargin (1 µM, 36 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ab16329 at 2 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green) in HCT 116 treated cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin 1/300. Panel d represents the merged image of HCT116 treated cells, which shows higher expression for TRAIL-R2 protein showing localization in nucleus, cytoplasm and membrane. Panel e represents the merged image of untreated HCT 116 cells, that shows lower or no expression for TRAIL-R2 protein. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
