Biotin Anti-Phosphotyrosine antibody [PY20]

• 产品名称

  生物素Anti-Phosphotyrosine抗体[PY20]
  参阅全部 Phosphotyrosine 一抗

• 描述

  生物素小鼠单克隆抗体[PY20] to Phosphotyrosine

• 宿主

  Mouse

• 偶联物

  Biotin

• 特异性

  Clone PY20 specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.

• 经测试应用

  适用于: IP, WB, ICCmore details

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Phosphotyrosine coupled to carrier protein

• 常规说明

  The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated proteins biochemical pathways in which they function.

  This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).

  The purified antibody is coupled through free primary amino groups to biotin. The binding site of the antibody was protected by the addition of phenyl phosphate prior to coupling.

  Source: Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.50
  Preservative: 0.08% Sodium azide
  Constituents: PBS, 65% Glycerol

• 纯度

  Protein A/G purified

• 克隆

  单克隆

• 克隆编号

  PY20

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab16388于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。