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Anti-HDAC4 antibody

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50ug
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产品详情
  • 产品名称

    Anti-HDAC4抗体
    参阅全部 HDAC4 一抗

  • 描述

    兔多克隆抗体to HDAC4

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-PWBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human HDAC4 aa 1-18.
    Sequence:

    MSSQSHPDGLSGRDQPVE

    Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: HeLa cell lysate. IHC-P: Human skin tissue.

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA

  • 浓度

    • 50 µg 浓度为 1 mg/ml

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab16339于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
IHC-P

1/20.

WB

1/1000. Predicted molecular weight: 140 kDa.

  • 数据库链接

  • 别名

    • HA6116 antibody

    • HD 4 antibody

    • HD4 antibody

    • HDAC 4 antibody

    • HDAC A antibody

    • HDAC4 antibody

    • HDAC4_HUMAN antibody

    • HDACA antibody

    • Histone deacetylase 4 antibody

    • Histone Deacetylase A antibody

    • KIAA0288 antibody

    • AHO3 antibody

    • BDMR antibody

    • EC 3.5.1.98 antibody

  • Western blot - Anti-HDAC4 antibody (ab16339)

    Western blot - Anti-HDAC4 antibody (ab16339)

    ab16339 at 1/1000 detecting HDAC4 from HeLa cell lysate by Western blot

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 antibody (ab16339)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 antibody (ab16339)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human skin tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Rabbit Polyclonal Antibody recognizing HDAC4 (ab16339 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.