Anti-HDAC4抗体
参阅全部 HDAC4 一抗
兔多克隆抗体to HDAC4
Rabbit
适用于: IHC-P, WBmore details
与反应: Human
Synthetic peptide corresponding to Human HDAC4 aa 1-18.
Sequence:
MSSQSHPDGLSGRDQPVE
WB: HeLa cell lysate. IHC-P: Human skin tissue.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab16339于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | 1/20. | |
WB | 1/1000. Predicted molecular weight: 140 kDa. |
Entrez Gene: 9759 Human
Omim: 605314 Human
SwissProt: P56524 Human
Unigene: 20516 Human
HA6116 antibody
HD 4 antibody
HD4 antibody
HDAC 4 antibody
HDAC A antibody
HDAC4 antibody
HDAC4_HUMAN antibody
HDACA antibody
Histone deacetylase 4 antibody
Histone Deacetylase A antibody
KIAA0288 antibody
AHO3 antibody
BDMR antibody
EC 3.5.1.98 antibody
Western blot - Anti-HDAC4 antibody (ab16339)
ab16339 at 1/1000 detecting HDAC4 from HeLa cell lysate by Western blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 antibody (ab16339)
Immunohistochemistry was performed on normal biopsies of deparaffinized Human skin tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Rabbit Polyclonal Antibody recognizing HDAC4 (ab16339 ) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.