Donkey Anti-Rabbit IgG H&L (HRP)

• 产品名称

  驴抗兔IgG H&L (HRP)
  参阅全部 IgG 二抗

• 宿主

  Donkey

• 靶标种属

  Rabbit

• 特异性

  Reacts with the heavy and light chains of the immunoglobulin molecule.

• 经测试应用

  适用于: WBmore details

• 免疫原

  Synthetic peptide corresponding to IgG.

• 偶联物

  HRP

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C.

• 存储溶液

  Preservative: 0.01% Thimerosal (merthiolate)
  Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA

• 浓度

• 250 µl 浓度为 0.04 mg/ml

• 纯度

  Protein G purified

• 克隆

  多克隆

• 同种型

  IgG

• 常规说明

  Part of the AbExcel range.

The Abpromise guarantee

Abpromise™承诺保证使用ab16284于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 

  Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

  All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution Developed using the ECL technique. Performed under reducing conditions. Exposure time : 150 seconds

• 

  ELISA - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

  ab16284 was used at dilution 1/5000 with the primary antibody ab52903 in ELISA. See the review on ab52903.

• 

  Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)

  ab16284 was used at dilution 1/2000 with the primary antibody ab28379 in WB. See the review on ab28379.