HRP Anti-Phosphotyrosine抗体[PY20]
参阅全部 Phosphotyrosine 一抗
HRP小鼠单克隆抗体[PY20] to Phosphotyrosine
Mouse
HRP
适用于: WBmore details
不适用于: IHC-P
与反应: Species independent
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
This antibody gave a positive signal in NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate.
This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
浓度
100 µl 浓度为 0.5 mg/ml
Immunogen affinity purified
单克隆
PY20
IgG2b
Abpromise™承诺保证使用ab16389于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/5000. |
应用说明
Is unsuitable for IHC-P.
Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)
HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 40 kDawhy is the actual band size different from the predicted?
Exposure time: 10 seconds
Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)
HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution ((BLOCKED WITH 3% MILK)) + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 40 kDawhy is the actual band size different from the predicted?
Exposure time: 2 minutes