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HRP Anti-Phosphotyrosine antibody [PY20]

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100uL
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产品详情
  • 产品名称

    HRP Anti-Phosphotyrosine抗体[PY20]
    参阅全部 Phosphotyrosine 一抗

  • 描述

    HRP小鼠单克隆抗体[PY20] to Phosphotyrosine

  • 宿主

    Mouse

  • 偶联物

    HRP

  • 经测试应用

    适用于: WBmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • This antibody gave a positive signal in NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate.

  • 常规说明

    This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • 存储溶液

    pH: 7.40
    Preservative: 0.1% Proclin 300 Solution
    Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)

  • 浓度

    • 100 µl 浓度为 0.5 mg/ml

  • 纯度

    Immunogen affinity purified

  • 克隆

    单克隆

  • 克隆编号

    PY20

  • 同种型

    IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab16389于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
WB

1/5000.

应用说明

Is unsuitable for IHC-P.

  • Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)

    Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)

    HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds

  • Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)

    Western blot - HRP Anti-Phosphotyrosine antibody [PY20] (ab16389)

    HRP Anti-Phosphotyrosine antibody [PY20] (ab16389) at 1/5000 dilution ((BLOCKED WITH 3% MILK)) + NIH 3T3 treated with Vanadate and PDGF Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes