Anti-Chromogranin A抗体
参阅全部 Chromogranin A 一抗
兔多克隆抗体to Chromogranin A
Rabbit
适用于: IHC-Pmore details
不适用于: WB
与反应: Human
预测可用于: Mouse, Monkey
Recombinant fragment within Human Chromogranin A aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P10645
IHC-P: FFPE human normal pancreas tissue
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15160于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (22) | 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
应用说明
Is unsuitable for WB.
Entrez Gene: 1113 Human
Entrez Gene: 12652 Mouse
Omim: 118910 Human
SwissProt: P10645 Human
SwissProt: P26339 Mouse
Unigene: 150793 Human
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
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CMGA_HUMAN antibody
ER-37 antibody
Pancreastatin antibody
Parastatin antibody
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Pituitary secretory protein I antibody
Secretory protein I antibody
SP I antibody
SP-I antibody
SP1 antibody
SPI antibody
vasostatin 2 antibody
Vasostatin antibody
Vasostatin I antibody
Vasostatin II antibody
vasostatin-2 antibody
beta Granin antibody
betagranin (N-terminal fragment of chromogranin A) antibody
catestatin antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (ab15160)
IHC image of Chromogranin A staining in a section of formalin-fixed paraffin-embedded normal human pancreas* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15160, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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