Anti-alpha Tubulin抗体- Microtubule Marker
参阅全部 alpha Tubulin 一抗
兔多克隆抗体to alpha Tubulin - Microtubule Marker
Rabbit
Homology to both Tubulin alpha-3E chain and Tubulin alpha-4A chain
适用于: WB, IHC-Pmore details
与反应: Human
预测可用于: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Pig, Xenopus laevis, Drosophila melanogaster, Zebrafish, Gerbil, Newt
Synthetic peptide within Human alpha Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q6PEY2
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15246于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (14) | Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa). |
| IHC-P | (1) | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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Unigene: 75318 Human
Unigene: 1155 Mouse
Unigene: 92961 Rat
Entrez Gene: 7277 Human
Entrez Gene: 22145 Mouse
Entrez Gene: 573122 Zebrafish
Entrez Gene: 573216 Zebrafish
Omim: 191110 Human
SwissProt: P68366 Human
SwissProt: P68368 Mouse
bA408E5.3 antibody
H2 ALPHA antibody
Hum a tub1 antibody
Hum a tub2 antibody
LIS3 antibody
MGC171407 antibody
MGC55332 antibody
TBA4A_HUMAN antibody
Testis-specific alpha-tubulin antibody
TUBA1 antibody
TUBA1A antibody
tuba1l antibody
Tuba4a antibody
Tubulin alpha 1 chain antibody
Tubulin alpha antibody
Tubulin alpha-1 chain antibody
tubulin alpha-1B chain antibody
Tubulin alpha-4A chain antibody
Tubulin H2-alpha antibody
Tubulin, alpha 1 (testis specific) antibody
tubulin, alpha 1, like antibody
Tubulin, alpha 4a antibody
Tubulin, alpha, testis-specific antibody
Tubulin, alpha-1 antibody
Alpha-tubulin 1 antibody
ALS22 antibody
B ALPHA 1 antibody
Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa
Lanes: 1-3: 4oC (1 freeze/thaw); 4-6: 4oC (2 freeze/thaws); 7-9 : 4oC (4 freeze/thaws)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
ab15246 at 1/200 dilution, staining alpha Tubulin in Human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections).
Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
All lanes : Anti-alpha Tubulin antibody - Microtubule Marker (ab15246) at 1 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 50 kDa
Observed band size: 55 kDawhy is the actual band size different from the predicted?
