WI-38核extract裂解物
参阅全部 WI 38 细胞裂解液
The protein content has been determined by a Bradford-based assay.
Yes
Liquid
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0002% Sodium molybdate, 0.216% Beta glycerophosphate, 0.018% Sodium orthovanadate, 0.042% Sodium fluoride, 0.015% DTT, 0.48% HEPES, 0.003% EDTA, 20% Glycerol, 2.34% Sodium chloride
With Protease inhibitor
浓度
250 µg 浓度为 2.5 mg/ml
Functional Studies - WI-38 nuclear extract lysate (ab14911)
Each lot has been tested for AP-1 activation by using TransAM™ AP-1 Kits. The signal intensity for AP-1 activation in each lot is compared to the signal intensity obtained with extracts from unstimulated WI-38 VA13 cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4 when using TransAM AP-1 c-Fos. This ratio may vary depending on the basal level of AP-1 activation in a given cell type.
