NIH/3T3全细胞裂解物(TPA stimulated)
参阅全部 NIH 3T3 细胞裂解液
Cell line: NIH/3T3 (Mouse embryonic fibroblast).
Cells were cultured in medium supplemented with 50 ng/ml TPA (phorbol, 12-myristate, 13 acetate) for 2 hours at 37°C immediately prior to harvesting.
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Yes
Liquid
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
pH: 6.80
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
浓度
200 µg 浓度为 5 mg/ml
