Anti-Bestrophin/BEST1抗体
参阅全部 Bestrophin/BEST1 一抗
兔多克隆抗体to Bestrophin/BEST1
Rabbit
适用于: IHC-Pmore details
与反应: Human
Synthetic peptide corresponding to Human Bestrophin/BEST1 aa 550-650 (C terminal).
Database link: O76090
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
浓度
50 µg 浓度为 0.75 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab14929于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Entrez Gene: 7439 Human
Omim: 607854 Human
SwissProt: O76090 Human
Unigene: 524910 Human
Unigene: 712676 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bestrophin/BEST1 antibody (ab14929)
IHC image of ab14929 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14929, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
