Anti-Mitotic proteins antibody [MPM-2]

• 产品名称

  Anti-Mitotic蛋白抗体[MPM-2]

• 描述

  小鼠单克隆抗体[MPM-2] to Mitotic蛋白

• 宿主

  Mouse

• 特异性

  Recognizes a phosphorylated epitope (S/T)P found in phosphoproteins such as MAP2, HSP70, cdc25 and DNA topoisomerase IIa, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM-2 varies from species to species and with the cell type. The clone number has been updated from (0.T.181) to (MPM-2) both clone numbers name the same antibody clone.

• 经测试应用

  适用于: IP, ICC/IF, ICC, ELISA, IHC-P, WB, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Human
  预测可用于: a wide range of other species

• 免疫原

  Tissue, cells or virus corresponding to Human Mitotic proteins.

• 阳性对照

• WB: Colcemid treated HeLa cell lysate. ICC/IF: Saos-2 cells. IHC-P: human normal skin tissue Flow Cyt (Intra): HeLa cells.

• 常规说明

  For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

  This product was changed from ascites to tissue culture supernatant on 11-June-19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.035% Sodium azide
  Constituents: 1.37% Tris glycine, 0.61% Sodium chloride, 30% Glycerol

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 纯化说明

  Purified from TCS.

• 克隆

  单克隆

• 克隆编号

  MPM-2

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab14581于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitotic proteins antibody [MPM-2] (ab14581)

  Ab14581 staining human normal skin. Staining is localised to the nucleus.
  Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  This image was generated using the ascites version of the product.

• 

  Immunocytochemistry - Anti-Mitotic proteins antibody [MPM-2] (ab14581)This image is courtesy of an anonymous Abreview

  ab14581 staining Mitotic proteins - Mitosis Marker in Human Saos-2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton in PBS and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500) for 1 hour. An Alexa Fluor® 594-conjugated Goat anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

  This image was generated using the ascites version of the product.

  See Abreview

• 

  Flow Cytometry (Intracellular) - Anti-Mitotic proteins antibody [MPM-2] (ab14581)

  Flow Cytometry analysis using anti-phospho-Ser/Thr-Pro, MPM-2 (ab14581). HeLa cells were treated with colcemid (green), or untreated (grey).

• 

  Western blot - Anti-Mitotic proteins antibody [MPM-2] (ab14581)

  All lanes : Anti-Mitotic proteins antibody [MPM-2] (ab14581) at 2 µg
  
  Lane 1 : Non treated HeLa cells
  Lane 2 : Colcemid-treated HeLa cells
  
  Lysates/proteins at 20 µg per lane.
  
  Predicted band size: 92 kDa
  
  

• 

  Western blot - Anti-Mitotic proteins antibody [MPM-2] (ab14581)

  All lanes : Anti-Mitotic proteins antibody [MPM-2] (ab14581) at 2 µg
  
  Lane 1 : Non-treated HeLa
  Lane 2 : Colcemid-treated HeLa
  
  Predicted band size: 92 kDa
  
  

• 

  Immunocytochemistry - Anti-Mitotic proteins antibody [MPM-2] (ab14581)

  ab14581 (green) labeling phospho-proteins in metaphase cells. The spindle, centrosomes and centromeric regions are stained. DNA is counterstained with propidium iodide (red).