Anti-Poly (ADP-Ribose) Polymer抗体
参阅全部 Poly (ADP-Ribose) Polymer 一抗
鸡多克隆抗体to Poly (ADP-Ribose) Polymer
Chicken
This antibody recognizes Poly (ADP-Ribose) Polymer synthesized by a variety of poly(ADP-ribose) polymerases (PARP)-related enzymes, including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. It does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA. It does cross-react to bovine serum albumin due to its use as a carrier for the immunogen.
适用于: WB, ELISAmore details
不适用于: ICC/IF,IHC-Fr or IHC-P
与反应: Species independent
Other Immunogen Type corresponding to Poly (ADP-Ribose) Polymer. Purified Poly (ADP-Ribose) Polymer mixed with methylated bovine serum albumin.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.316% Tris HCl, 0.87% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
IgY fraction
多克隆
IgY
Abpromise™承诺保证使用ab14460于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) | Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 1 kDa. |
ELISA | Use at an assay dependent concentration. It is recommended to start with a 1/100 dilution. |
应用说明
Is unsuitable for ICC/IF,IHC-Fr or IHC-P.
pADPr antibody
Poly ADP ribose antibody
Western blot - Anti-Poly (ADP-Ribose) Polymer antibody (ab14460)
Western blot analysis of Poly (ADP-Ribose) Polymer. Lane 1: control HL60 cells (75,000 cells). Lane 2: cells automodified with PARP1. Note the apparent shift in molecular weight PARP1 from 116kDa to a broad range >250kDa due to various extents of Poly (ADP-Ribose) Polymer automodification. ab14460 was used at a concentration of 10 µg/ml.