Anti-TRF1 antibody [3H11]

• 产品名称

  Anti-TRF1抗体[3H11]
  参阅全部 TRF1 一抗

• 描述

  小鼠单克隆抗体[3H11] to TRF1

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, WB, ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Human TRF1 protein.

• 阳性对照

• HeLa cell nuclear lysate

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 99.98% PBS

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  3H11

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab14397于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7013 Human

• Omim: 600951 Human

• SwissProt: P54274 Human

• Unigene: 442707 Human

• 别名

• PIN 2 antibody

• PIN2 antibody

• t TRF1 antibody

• Telomeric protein Pin2 antibody

• Telomeric protein Pin2/TRF1 antibody

• Telomeric repeat binding factor (NIMA interacting) 1 antibody

• Telomeric repeat binding factor 1 antibody

• Telomeric repeat binding protein 1 antibody

• Telomeric repeat-binding factor 1 antibody

• TERF 1 antibody

• Terf1 antibody

• TERF1_HUMAN antibody

• TRBF 1 antibody

• TRBF1 antibody

• TRF 1 antibody

• TRF antibody

• TTAGGG repeat binding factor 1 antibody

• TTAGGG repeat-binding factor 1 antibody

• hTRF1 AS antibody

• NIMA interacting protein 2 antibody

• NIMA-interacting protein 2 antibody

• 

  Western blot - Anti-TRF1 antibody [3H11] (ab14397)

  Anti-TRF1 antibody [3H11] (ab14397) at 1/200 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Predicted band size: 50 kDa
  Observed band size: 55 kDawhy is the actual band size different from the predicted?
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-TRF1 antibody [3H11] (ab14397)

  ICC/IF image of ab14397 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14397, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Flow Cytometry - Anti-TRF1 antibody [3H11] (ab14397)

  Overlay histogram showing HeLa cells stained with ab14397 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14397, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.