Anti-PDE4D antibody

• 产品名称

  Anti-PDE4D抗体
  参阅全部 PDE4D 一抗

• 描述

  兔多克隆抗体to PDE4D

• 宿主

  Rabbit

• 特异性

  ab14613 labels all PDE4D proteins including PDE4D1, PDE4D2, PDE4D3, PDE4D4, PDE4D5 variants. Does not cross react with PDE4A, PDE4B, PDE4D6, PDE4D7, PDE4D8, PDE4D9 or PDE4C. The antibody has not been tested against endogenous PDE4D, only recombinant proteins and we therefore cannot guarantee it will detect endogenous PDE4.

• 经测试应用

  适用于: WB, IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide

• 浓度

• 批次浓度范围 50 µg 浓度为 0.7 - 0.78 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab14613于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5144 Human

• Omim: 600129 Human

• SwissProt: Q08499 Human

• Unigene: 117545 Human

• Unigene: 721404 Human

• 别名

• cAMP specific phosphodiesterase 4D antibody

• cAMP specific phosphodiesterase PDE4D6 antibody

• cAMP-specific 3'' antibody

• DPDE3 antibody

• Dunce like phosphodiesterase E3 antibody

• FLJ97311 antibody

• HSPDE4D antibody

• PDE43 antibody

• Pde4d antibody

• PDE4D_HUMAN antibody

• PDE4DN2 antibody

• Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody

• Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody

• Phosphodiesterase 4D cAMP specific antibody

• Phosphodiesterase 4D, cAMP specific (dunce) antibody

• STRK1 antibody

• cAMP specific 3',5' cyclic phosphodiesterase 4D antibody

• DKFZp686M11213 antibody

• 5''-cyclic phosphodiesterase 4D antibody

• 

  Western blot - Anti-PDE4D antibody (ab14613)

  Western blot using ab14613 and positive controls for PDE4D variants.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4D antibody (ab14613)

  ab14613 (2µg/ml) staining PDE4D in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm.
  Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.