Anti-FKBP15抗体
Western blot - Anti-FKBP135 antibody (ab14432)
All lanes : Anti-FKBP15 antibody (ab14432) at 0.1 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 135 kDa
Exposure time: 3 minutes
Western blot - Anti-FKBP135 antibody (ab14432)
All lanes : Anti-FKBP15 antibody (ab14432) at 0.2 µg/ml
Lane 1 : Nuclear lysates prepared from Hela cells at 5 µg
Lane 2 : Nuclear lysates prepared from Hela cells at 20 µg
Lanes 3-5 : Nuclear lysates prepared from Hela cells at 1 mg/ml
Predicted band size: 135 kDa
Antibodies: ab14432 used at 0.2 mcg/ml for WB in A and B and 2 mcg/mg extract for IP (B), ab14433 and ab14434 used at 2 mcg/mg extract for IP. Detection: Chemiluminescence with an exposure time of 25 minutes (A) and 10 minutes (B).
In figure B the Immunoprecipitation was done using ab14432 (Lane 3), ab14433 (Lane 4) and ab14434 (Lane 5) at 2µg/mg of extract.
兔多克隆抗体to FKBP15
Rabbit
适用于: WB, IPmore details
与反应: Mouse, Human
预测可用于: Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human FKBP135 aa 50-150.
Database link: Q5T1M5
WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibody was purified using an epitope specific to FKBP-15 immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab14432于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 135 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Entrez Gene: 23307 Human
SwissProt: Q5T1M5 Human
Unigene: 522351 Human
FKB15_HUMAN antibody
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WASP and FKBP-like antibody
133 kDa FK506-binding protein antibody
133 kDa FKBP antibody
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