Anti-Periostin抗体
参阅全部 Periostin 一抗
兔多克隆抗体to Periostin
Rabbit
适用于: ELISA, ICC/IF, IHC-Fr, IHC-Pmore details
与反应: Mouse, Rat, Chicken, Human
Recombinant fragment (His-tag) corresponding to Human Periostin (N terminal).
IHC-P: Human colon and normal skin tissues; Prostate cancer tissues. ICC/IF: HeLa cells.
Lots # GR310924-1, GR296166-4, and GR296166-5 are sold in lyophilized form. To reconstitute, add 100 µL of deionized water and let the lyophilized antibody dissolve completely. The resulting concentration is 0.5 mg/mL. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, 0.58% Sodium chloride
浓度
50 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab14041于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | (3) | Use at an assay dependent concentration. |
| IHC-Fr | (6) | Use at an assay dependent concentration. PubMed: 19079190 |
| IHC-P | (8) | 1/100 - 1/1000. |
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Unigene: 236067 Mouse
Entrez Gene: 10631 Human
Entrez Gene: 50706 Mouse
Omim: 608777 Human
SwissProt: Q15063 Human
SwissProt: Q62009 Mouse
Unigene: 136348 Human
Unigene: 721018 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab14041)
Immunohistochemistry analysis of formalin-fixed paraffin-embedded human colon tissue section labeling Periostin with ab14041.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab14041)
Immunohistochemistry analysis of formalin-fixed paraffin-embedded human normal skin tissue sectionlabeling Periostin with ab14041.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (ab14041)Tian et al. PLoS One. 2015 Mar 17;10(3):e0121502. doi: 10.1371/journal.pone.0121502. eCollection 2015. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
The periostin staining in prostate cancer and paired non-tumorous prostate tissues (TMA from JHU, 140 cases).
(A) The pathologists’ criteria for IHC scoring.(a) 0, undetectable; (b)1+, weak staining; (c) 2+, medium staining; (d) 3+, strong staining. Periostin was mainly stained in peritumoral stroma.
Immunocytochemistry/ Immunofluorescence - Anti-Periostin antibody (ab14041)
ICC/IF image of ab14041 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in 100% methanol (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14041, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
