Anti-Poly (ADP-Ribose) Polymer抗体[10H]
参阅全部 Poly (ADP-Ribose) Polymer 一抗
小鼠单克隆抗体[10H] to Poly (ADP-Ribose) Polymer
Mouse
This antibody reacts with poly (ADP-Ribose) Polymer synthesized by a variety of poly(ADP- ribose) polymerases (PARP)-related enzymes including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. The antibody does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA.
适用于: IHC-Frmore details
与反应: Species independent
Other Immunogen Type conjugated to bovine serum albumin. Poly (ADP-Ribose) polymer mixed with methylated bovine serum albumin.
Rat liver induced for Poly (ADP-Ribose) Polymer synthesis by injection with diethylnitrosamine. MEF's treated with 500um Hydrogen Peroxide.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.242% Tris, 0.87% Sodium chloride, 1% BSA
浓度
100 µg 浓度为 1 mg/ml
Protein A purified
单克隆
10H
NS1
IgG3
kappa
Abpromise™承诺保证使用ab14459于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-Fr | (1) | Use at an assay dependent concentration |
Immunohistochemistry (Frozen sections) - Anti-Poly (ADP-Ribose) Polymer antibody [10H] (ab14459)
Immunohistochemistry of rat livers treated with diethylnitrosamine (200 mg/kg) and stained with ab14459 diluted 1/100. After treatment livers were removed and rapidly processed 10 hr later, at peak polymer induction. Left hand side image was from diethylnitrosamine untreated liver tissue and right one represents DEN treated sections.