Anti-TLR5抗体[19D759.2]
参阅全部 TLR5 一抗
小鼠单克隆抗体[19D759.2] to TLR5
Mouse
This antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5.
适用于: IP, ICC, WB, Flow Cyt (Intra), ICC/IF, IHC-P, IHC-Frmore details
与反应: Mouse, Human
Synthetic peptide: SSKDFTWVQN ALLKHLDTQY SDQNRFNLCF EERDFVPGEN RIANIQDAIW NSRKIVCLVS RHFLRDGWCL EAFSYAQGRC LSDLNSALIM VVVGSLSQY QL conjugated to KLH, corresponding to amino acids 700-800 of Human TLR5.
Flow Cyt (Intra): Ramos cells WB: Ramos and Raw cell lysate.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Protein G purified
单克隆
19D759.2
IgG2a
kappa
Abpromise™承诺保证使用ab13876于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (2) | Use at an assay dependent concentration. |
| ICC | (1) | Use at an assay dependent concentration. |
| WB | (2) | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 103 kDa. |
| Flow Cyt (Intra) | Use 2µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody | |
| ICC/IF | (1) | Use at an assay dependent concentration. |
| IHC-P | (2) | Use at an assay dependent concentration. Antigen retrieval with citrate buffer pH 6. |
| IHC-Fr | (1) | Use at an assay dependent concentration. (see Miller et al). |
Entrez Gene: 7100 Human
Entrez Gene: 53791 Mouse
Omim: 603031 Human
SwissProt: O60602 Human
SwissProt: Q9JLF7 Mouse
Unigene: 604542 Human
Unigene: 116894 Mouse
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![Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (ab13876)](https://www.abcam.cn/ps/products/13/ab13876/Images/ab13876-21362-anti-tlr5-antibody-19d7592-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (ab13876)
Flow Cytometry (Intracellular) analysis of TLR5 in 106 Ramos cells using (A) 2µg of isotype control and (B) 2µg of ab13876
![Western blot - Anti-TLR5 antibody [19D759.2] (ab13876)](https://www.abcam.cn/ps/products/13/ab13876/Images/ab13876-21363-anti-tlr5-antibody-19d7592-western-blot.jpg)
Western blot - Anti-TLR5 antibody [19D759.2] (ab13876)
All lanes : Anti-TLR5 antibody [19D759.2] (ab13876) at 2 µg/ml
Lane 1 : Ramos cell lysate
Lane 2 : Raw cell lysate
Predicted band size: 103 kDa
Western blot analysis of TLR5 in human Ramos cell lysate (A) and mouse Raw cell,lysate (B) using ab13876 at 2µg/ml
