Anti-beta Defensin 1 antibody [M11-14b-D10]

• 产品名称

  Anti-beta Defensin 1抗体[M11-14b-D10]
  参阅全部 beta Defensin 1 一抗

• 描述

  小鼠单克隆抗体[M11-14b-D10] to beta Defensin 1

• 宿主

  Mouse

• 经测试应用

  适用于: WB, ELISA, IHC-Pmore details

• 种属反应性

  与反应: Human
  预测可用于: Chimpanzee, Baboon, Macaque monkey

• 免疫原

  Synthetic peptide corresponding to Human beta Defensin 1 aa 1-100.

  Run BLAST with  Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Constituent: 0.79% Tris HCl

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Protein L purified

• 克隆

  单克隆

• 克隆编号

  M11-14b-D10

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab14425于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1672 Human

• Omim: 602056 Human

• SwissProt: P60022 Human

• Unigene: 32949 Human

• 别名

• beta 1 antibody

• Beta defensin 1 antibody

• Beta-defensin 1 antibody

• DEFB 1 antibody

• DEFB1 antibody

• DEFB1_HUMAN antibody

• DEFB101 antibody

• Defensin antibody

• Defensin beta 1 antibody

• Defensin beta 1 preproprotein antibody

• HBD 1 antibody

• hBD-1 antibody

• HBD1 antibody

• MGC51822 antibody

• BD 1 antibody

• BD-1 antibody

• BD1 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Defensin 1 antibody [M11-14b-D10] (ab14425)

  ab14425 (2µg/ml) staining beta defensin in human kidney, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining in the epithelial layers of the loops of Henle, distal tubules and collecting ducts.
  Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.