Anti-DDX4 / MVH抗体
参阅全部 DDX4 / MVH 一抗
兔多克隆抗体to DDX4 / MVH
Rabbit
适用于: WB, ICC/IF, IHC-P, IHC-Frmore details
与反应: Mouse, Human
预测可用于: Rat, Sheep, Cow, Pig, Platypus
Synthetic peptide within Human DDX4/ MVH aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9NQI0
(Peptide available as ab13841)
ICC: mES cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 1 - 1.03 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab13840于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). |
| ICC/IF | (2) | Use a concentration of 1 µg/ml. |
| IHC-P | (11) | Use at an assay dependent concentration. |
| IHC-Fr | (7) | Use at an assay dependent concentration. |
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Unigene: 223581 Human
Unigene: 12818 Mouse
Unigene: 198577 Rat
Entrez Gene: 54514 Human
Entrez Gene: 13206 Mouse
Omim: 605281 Human
SwissProt: Q9NQI0 Human
SwissProt: Q61496 Mouse
DEAD (Asp Glu Ala Asp) box polypeptide 4 antibody
DEAD box protein 4 antibody
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody
MVH antibody
Probable ATP dependent RNA helicase DDX4 antibody
Probable ATP-dependent RNA helicase DDX4 antibody
VASA antibody
Vasa homolog antibody
DDX 4 antibody
Ddx4 antibody
DDX4_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-DDX4 / MVH antibody (ab13840)
ab13840 staining DDX4/MVH in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab13840 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody (ab13840)This image is courtesy of Petra Hajkova, Gurdon Institute, University of Cambridge
Mouse embryonic gonad cells were fixed with 4% PFA, permeabilised and blocked with PBS, 1% BSA, 0.1% TX-100 and incubated with the DDX4 antibody, ab13840 (1 µg/ml) overnight at 4 degrees. ab13840 staining is shown in red and Oct4 staining is shown in green.
The image shows that the anti-DDX4 antibody, ab13840, stains the cytoplasm of mouse primordial germ cells when used in IHC-Fr.
Western blot - Anti-DDX4 / MVH antibody (ab13840)All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Additional bands at: 28 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-DDX4 / MVH antibody (ab13840)All lanes : ab13840 at 1 µg/ml
Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
Lane 2 : Human testis tissue lysate - total protein
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 79 kDawhy is the actual band size different from the predicted?
Additional bands at: 47 kDa, 52 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-DDX4 / MVH antibody (ab13840)All lanes : Anti-DDX4 / MVH antibody (ab13840) at 1 µg/ml
Lane 1 : Mouse Ovary Lysate at 10 µg
Lane 2 : Mouse Embryonic Germ Cell Lysate at 20 µg
Lane 3 : Mouse Ovary Lysate at 10 µg with DDX4 / MVH peptide (ab13841) at 1 µg/ml
Lane 4 : Mouse Embryonic Germ Cell Lysate at 20 µg with DDX4 / MVH peptide (ab13841) at 1 µg/ml
Predicted band size: 76 kDa
Observed band size: 76 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (ab13840)IHC image of DDX4/MVH staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13840, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
