Anti-Alpha B Crystallin抗体
参阅全部 Alpha B Crystallin 一抗
兔多克隆抗体to Alpha B Crystallin
Rabbit
Does not cross-react with αA-crystallin.
适用于: IP, WB, ICC, IHC-Fr, IHC-P, ICC/IFmore details
与反应: Mouse, Rat, Chicken, Cow, Human, Pig
预测可用于: Rabbit, Hamster
Synthetic peptide:
REEKPAVTAAPKK
conjugated to KLH, corresponding to amino acids 163-175 of Human alpha B Crystallin.
ICC: HeLa. (Heat shocked). WB: A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 , and Rat brain cell lysate.
For maximum product recovery, after thawing, centrifuge the product vial before removing cap
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.1% PBS
浓度
100 µl 浓度为 1 mg/ml
Affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab13497于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | (3) | 1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa). |
| ICC | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | (1) | Use at an assay dependent concentration. |
| ICC/IF | 1/120. |
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SwissProt: Q05713 Chicken
SwissProt: P02511 Human
SwissProt: P23927 Mouse
SwissProt: P41316 Rabbit
Unigene: 53454 Human
Unigene: 178 Mouse
Unigene: 98208 Rat
Entrez Gene: 396089 Chicken
Entrez Gene: 1410 Human
Entrez Gene: 12955 Mouse
Entrez Gene: 100009295 Rabbit
Omim: 123590 Human
Alpha(B)-crystallin antibody
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Crystallin alpha polypeptide 2 antibody
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Heat shock 20 kD like protein antibody
Heat shock protein beta 5 antibody
Heat shock protein beta-5 antibody
HspB5 antibody
Renal carcinoma antigen NY REN 27 antibody
Renal carcinoma antigen NY-REN-27 antibody
Rosenthal fiber component antibody
AACRYA antibody
Alpha B crystallin antibody
Alpha crystallin B chain antibody
Western blot - Anti-Alpha B Crystallin antibody (ab13497)
Anti-Alpha B Crystallin antibody (ab13497) at 1/1000 dilution + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 20 kDa
Observed band size: 22 kDawhy is the actual band size different from the predicted?
Western blot - Anti-Alpha B Crystallin antibody (ab13497)
Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497).
Lane 1: Molecular Weight Ladder (MW).
Lane 2: A431 cell lysates.
Lane 3: HCT116 cell lysates.
Lane 4: HeLa cell lysates.
Lane 5: HepG2 cell lysates.
Lane 6: HEK293 cell lysates.
Lane 7: HUVEC cell lysates.
Lane 8: Jurkat cell lysates.
Lane 9: MCF7 cell lysates.
Lane 10: PC3 cell lysates.
Lane 11: T98G cell lysates.
Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497) at 1/1000 dilution for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/1000 dilution for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.
Western blot - Anti-Alpha B Crystallin antibody (ab13497)
Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497).
Lane 1: Molecular Weight Ladder (MW).
Lane 2: Rat Brain cell lysates.
Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ab13497) at 1/1000 dilution for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/1000 dilution for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody (ab13497)
Immunofluorescent analysis of 2% paraformaldehyde fixed Heat Shocked (at 42°C for 1h) Cervical cancer cell line (HeLa) for 20min at RT, labelling Alpha B Crystallin with ab13497 at 1/120 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (FITC) (Green) at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT. Image showing actin filament bundles, nuclear splicing speckles, and Exosome staining. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody (green). (C) Composite image.
Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody (ab13497)
Immunofluorescent analysis of 2% paraformaldehyde fixed Heat Shocked (at 42°C for 1h) Cervical cancer cell line (HeLa) for 20min at RT, labelling Alpha B Crystallin with ab13497 at 1/120 dilution for 12 hours at 4°C, followed by secondary antibody Goat Anti-Rabbit (APC) (Red) at 1/200 dilution for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at RT. Image showing actin filament bundles, nuclear splicing speckles, and Exosome staining. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody (Red). (C) Composite image.
