Anti-Superoxide Dismutase 1抗体
参阅全部 Superoxide Dismutase 1 一抗
兔多克隆抗体to Superoxide Dismutase 1
Rabbit
适用于: IHC-P, ICC/IF, WBmore details
与反应: Human
Synthetic peptide corresponding to Rat Superoxide Dismutase 1.
Database link: P07632
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
This antibody was purified on an antigen coupled sepharose column.
多克隆
IgG
Abpromise™承诺保证使用ab13499于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
ICC/IF | 1/120. | |
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 19, 23 kDa (predicted molecular weight: 18 kDa). |
Entrez Gene: 6647 Human
Omim: 147450 Human
SwissProt: P00441 Human
Unigene: 443914 Human
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Western blot - Anti-Superoxide Dismutase 1 antibody (ab13499)
Anti-Superoxide Dismutase 1 antibody (ab13499) at 1 µg/ml + Cell lysates prepared from mixed human cell lines (A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G)
Predicted band size: 18 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab13499)
Ab13499 staining Human normal colon. Staining is localized to the nucleus.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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