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Anti-Superoxide Dismutase 1 antibody

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50ug
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产品详情
  • 产品名称

    Anti-Superoxide Dismutase 1抗体
    参阅全部 Superoxide Dismutase 1 一抗

  • 描述

    兔多克隆抗体to Superoxide Dismutase 1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-PICC/IFWBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Rat Superoxide Dismutase 1.
    Database link: 
    P07632

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)

  • 浓度

    • 50 µg 浓度为 1 mg/ml

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    This antibody was purified on an antigen coupled sepharose column.

  • 克隆

    多克隆

  • 同种型

    IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab13499于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
IHC-P

Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ICC/IF

1/120.

WB

Use a concentration of 1 µg/ml. Detects a band of approximately 19, 23 kDa (predicted molecular weight: 18 kDa).

  • 数据库链接

  • 别名

    • Cu/Zn SOD antibody

    • Cu/Zn superoxide dismutase antibody

    • Epididymis secretory protein Li 44 antibody

    • HEL S 44 antibody

    • Homodimer antibody

    • hSod1 antibody

    • Indophenoloxidase A antibody

    • IPOA antibody

    • Mn superoxide dismutase antibody

    • SOD antibody

    • SOD soluble antibody

    • SOD1 antibody

    • SOD2 antibody

    • SODC antibody

    • SODC_HUMAN antibody

    • Superoxide dismutase [Cu-Zn] antibody

    • Superoxide dismutase 1 antibody

    • Superoxide dismutase 1 soluble antibody

    • Superoxide dismutase Cu Zn antibody

    • Superoxide dismutase cystolic antibody

    • ALS antibody

    • ALS1 antibody

    • Amyotrophic lateral sclerosis 1 adult antibody

  • Western blot - Anti-Superoxide Dismutase 1 antibody (ab13499)

    Western blot - Anti-Superoxide Dismutase 1 antibody (ab13499)

    Anti-Superoxide Dismutase 1 antibody (ab13499) at 1 µg/ml + Cell lysates prepared from mixed human cell lines (A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G)

    Predicted band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab13499)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Superoxide Dismutase 1 antibody (ab13499)

    Ab13499 staining Human normal colon. Staining is localized to the nucleus.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.


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